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Survey of the Effect of Doxorubicin and Flavonoid Extract of White Morus Alba Leaf on Apoptosis Induction in A-172 Gbm Cell Line Publisher Pubmed



Dabili S1 ; Fallah S1 ; Aein M1 ; Vatannejad A2 ; Panahi G2 ; Fadaei R2 ; Moradi N1 ; Shojaii A3
Authors
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Authors Affiliations
  1. 1. Department of Biochemistry, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Biochemistry, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Medicinal Plants, Research Institute for Islamic and Complementary Medicine, Iran University of Medical Sciences, Tehran, Iran

Source: Archives of Physiology and Biochemistry Published:2019


Abstract

In this study, the effect of doxorubicin, flavonoid extract of white Morus alba leaf (MFE) and a combination of doxorubicin and flavonoid extract on Bax and Bcl2 levels and caspase 3 activity of cancer A-172 GBM cell line was investigated. Bax/Bcl2 levels of treated A-172 GBM cell line with flavonoid extract of white mulberry leaf were estimated by ELISA methods. Caspase 3 activity of treated A-172 GBM cells was determined by calorimetric assay. The flow cytometry assessment was used to estimate the apoptosis percent of treated A-172 GBM cells. Treatment of A-172 GBM cells with MFE, doxorubicin and a combination of MFE and doxorubicin caused a significant decrease in Bcl2 level and an increase in Bax level. The apoptosis percent of treated cells were also elevated significantly. Present results suggest that concomitant use of herbal medicine and chemotherapy may be an effective alternative method for the treatment of cancers. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.
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