Quantification of Flavonoids in Alpinia Officinarum Hance. Via Hplc and Evaluation of Its Cytotoxicity on Human Prostate Carcinoma (Lncap) and Breast Carcinoma (Mcf-7) Cells

Quantification of Flavonoids in Alpinia Officinarum Hance. Via Hplc and Evaluation of Its Cytotoxicity on Human Prostate Carcinoma (Lncap) and Breast Carcinoma (Mcf-7) Cells Publisher Pubmed

Kazemi S¹ ; Asadi F² ; Barari L¹ ; Morakabati P¹ ; Jahani M³ ; Kani SNM¹ ; Soorani F⁴ ; Kolangi F⁵ ; Memariani Z⁶

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1. Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
2. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
4. Department of Pharmacology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
5. Department of Traditional Medicine, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
6. Traditional Medicine and History of Medical Sciences Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran

Source: Anti-Cancer Agents in Medicinal Chemistry Published:2022

Abstract

Background: Various plant species have been shown to be effective in the prevention or adjuvant therapy of cancer. Alpinia officinarum and its main phytochemicals have also been the subject of several studies for their anti-cancer properties. Objective: The objective of this study is to analyze the extracts of A. officinarum to quantify flavonoids and to evaluate the growth inhibitory effects of the extracts on MCF-7 and LNCaP cells. Methods: A. officinarum aqueous and hydroalcoholic extracts were analyzed by using High-Performance Liquid Chro-matography (HPLC) for the quantification of three flavonoid compounds. Then, MCF-7, LNCaP, and fibroblast cells were treated with several concentrations (25, 50, 100, 200, and 400 µg/mL) of extracts (24, 48 and 72h). Cell viability was assessed using an MTT assay. Flow cytometry was conducted to evaluate apoptosis. Results: Galangin and kaempferol (3.85 and 1.57 mg/g dry extract) were quantified, respectively, in hydroalcoholic and aqueous extracts using a validated method. The hydroalcoholic extract significantly decreased the viability of MCF-7 (IC50: 43.45µg/mL for 48h) and LNCaP cells (IC50: 168 µg/mL for 48h). The aqueous extract reduced cancer cell viability by more than 50% only at 200 and 400 µg/mL (72 h). Treatment of primary fibroblasts with both extracts showed no significant decrease in cell viability (25-100 µg/mL; 24 and 48h). The hydroalcoholic extract induced a significant increase in apoptotic cells in both MCF-7 and LNCaP cells. Conclusion: Obtained results demonstrated the cytotoxicity of A. officinarum through apoptosis induction in two cancer cell lines. Further investigations are required to determine the underlying apoptotic cell death mechanisms induced by A. officinarum in cancerous cells. © 2022 Bentham Science Publishers.

Style	Citing Format
MLA	Kazemi S, et al.. "Quantification of Flavonoids in Alpinia Officinarum Hance. Via Hplc and Evaluation of Its Cytotoxicity on Human Prostate Carcinoma (Lncap) and Breast Carcinoma (Mcf-7) Cells." Anti-Cancer Agents in Medicinal Chemistry, vol. 22, no. 4, 2022, pp. 721-730.
APA	Kazemi S, Asadi F, Barari L, Morakabati P, Jahani M, Kani SNM, Soorani F, Kolangi F, Memariani Z (2022). Quantification of Flavonoids in Alpinia Officinarum Hance. Via Hplc and Evaluation of Its Cytotoxicity on Human Prostate Carcinoma (Lncap) and Breast Carcinoma (Mcf-7) Cells. Anti-Cancer Agents in Medicinal Chemistry, 22(4), 721-730.
Chicago	Kazemi S, Asadi F, Barari L, Morakabati P, Jahani M, Kani SNM, Soorani F, Kolangi F, Memariani Z. "Quantification of Flavonoids in Alpinia Officinarum Hance. Via Hplc and Evaluation of Its Cytotoxicity on Human Prostate Carcinoma (Lncap) and Breast Carcinoma (Mcf-7) Cells." Anti-Cancer Agents in Medicinal Chemistry 22, no. 4 (2022): 721-730.
Harvard	Kazemi S et al. (2022) 'Quantification of Flavonoids in Alpinia Officinarum Hance. Via Hplc and Evaluation of Its Cytotoxicity on Human Prostate Carcinoma (Lncap) and Breast Carcinoma (Mcf-7) Cells', Anti-Cancer Agents in Medicinal Chemistry, 22(4), pp. 721-730.
Vancouver	Kazemi S, Asadi F, Barari L, Morakabati P, Jahani M, Kani SNM, et al.. Quantification of Flavonoids in Alpinia Officinarum Hance. Via Hplc and Evaluation of Its Cytotoxicity on Human Prostate Carcinoma (Lncap) and Breast Carcinoma (Mcf-7) Cells. Anti-Cancer Agents in Medicinal Chemistry. 2022;22(4):721-730.
BibTex	@article{ author = {Kazemi S and Asadi F and Barari L and Morakabati P and Jahani M and Kani SNM and Soorani F and Kolangi F and Memariani Z}, title = {Quantification of Flavonoids in Alpinia Officinarum Hance. Via Hplc and Evaluation of Its Cytotoxicity on Human Prostate Carcinoma (Lncap) and Breast Carcinoma (Mcf-7) Cells}, journal = {Anti-Cancer Agents in Medicinal Chemistry}, volume = {22}, number = {4}, pages = {721-730}, year = {2022} }
RIS	TY - JOUR AU - Kazemi S AU - Asadi F AU - Barari L AU - Morakabati P AU - Jahani M AU - Kani SNM AU - Soorani F AU - Kolangi F AU - Memariani Z TI - Quantification of Flavonoids in Alpinia Officinarum Hance. Via Hplc and Evaluation of Its Cytotoxicity on Human Prostate Carcinoma (Lncap) and Breast Carcinoma (Mcf-7) Cells JO - Anti-Cancer Agents in Medicinal Chemistry VL - 22 IS - 4 SP - 721 EP - 730 PY - 2022 ER -

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