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Antitumor Effects of a Recombinant Baculovirus Displaying Anti-Her2 Scfv Expressing Apoptin in Her2 Positive Sk-Br-3 Breast Cancer Cells Publisher



Meysami P1 ; Rezaei F1 ; Marashi SM1 ; Amiri MM2 ; Bakker E3 ; Mokhtariazad T1
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Authors Affiliations
  1. 1. Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, 1471613151, Iran
  2. 2. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, 1471613151, Iran
  3. 3. School of Medicine, University of Central Lancashire, Preston, United Kingdom

Source: Future Virology Published:2019


Abstract

Aim: Since HER2 targeted therapies have shown clinical benefit in breast cancer, in the present study recombinant baculovirus (BV) displaying anti-HER2 single-chain variable domain fragment (scFv) expressing Apoptin was generated. Methods: The binding specificity and surface display of anti-HER2 scFv were evaluated using enzyme-linked immunosorbent assay (ELISA) and electron microscopy, respectively. The targeting properties and cytotoxic effect on breast cancer cells determined by fluorescence microscopy and MTT assays. Results: The results demonstrated that recombinant BV could specifically bind to HER2-overexpressing SK-BR-3 cells but not to the HER2 negative MCF-7 cells and reduced the viability of SK-BR-3 cells by expressing Apoptin. Conclusion: These results suggest that the antitumor effect of Apoptin in combination with HER2 targeting of this recombinant BV makes it a promising vector in targeted cancer therapy. © 2019 Future Medicine Ltd.
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