Molecular Typing of Cytotoxin-Producing Klebsiella Oxytoca Isolates by 16S-23S Internal Transcribed Spacer Pcr

Molecular Typing of Cytotoxin-Producing Klebsiella Oxytoca Isolates by 16S-23S Internal Transcribed Spacer Pcr Publisher

Soltan Dallal MM^{1, 2} ; Validi M^{3, 4} ; Douraghi M^{2, 4} ; Bakhshi B⁵

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1. Department of Food Microbiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2. Food Microbiology Research Centre, Tehran University of Medical Sciences, Tehran, Iran
3. Clinical Biochemistry Research Centre, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
4. Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
5. Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Source: New Microbes and New Infections Published:2019

Abstract

Cytotoxin is one of the important pathogenic factors, which plays a role in the virulence of Klebsiella oxytoca. The aim of this study was to investigate molecular typing of clinical isolates of the cytotoxin-producing K. oxytoca using internal transcribed spacer (ITS)PCR. A total of 75 isolates of K. oxytoca were isolated from clinical samples; they were verified as K. oxytoca by standard microbiological tests and PCR. Production of toxin determines the cytotoxic effects on HEp-2 cells. The genetic diversity of isolates of the cytotoxin-producing K. oxytoca were defined by ITS-PCR. Of all the isolates investigated, five K. oxytoca strains isolated from stool cultures, two strains from blood samples, one strain from a wound and one strain isolated from urine had cytotoxic effects on HEp-2 cells. The ITS-PCR patterns showed genetic diversity among cytotoxin-producing isolates. The ITS-PCR method had good discriminatory power; performance of this method and interpretation of the results were easy and repeatable. Five genetic diversity patterns were identified by ITS-PCR. © 2019 The Author(s)

2. Identification of Cytotoxin-Producing Klebsiella Oxytoca Strains Isolated From Clinical Samples With Cell Culture Assays, Microbial Pathogenesis (2017)

3. Identification of Klebsiella Pneumoniae Carbapenemase-Producing Klebsiella Oxytoca in Clinical Isolates in Tehran Hospitals, Iran by Chromogenic Medium and Molecular Methods, Osong Public Health and Research Perspectives (2016)

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Masoumeh Douraghi (3)

Mohammad Mehdi Soltan Dallal (3)

Style	Citing Format
MLA	Soltan Dallal MM, et al.. "Molecular Typing of Cytotoxin-Producing Klebsiella Oxytoca Isolates by 16S-23S Internal Transcribed Spacer Pcr." New Microbes and New Infections, vol. 30, no. , 2019, pp. -.
APA	Soltan Dallal MM, Validi M, Douraghi M, Bakhshi B (2019). Molecular Typing of Cytotoxin-Producing Klebsiella Oxytoca Isolates by 16S-23S Internal Transcribed Spacer Pcr. New Microbes and New Infections, 30(), -.
Chicago	Soltan Dallal MM, Validi M, Douraghi M, Bakhshi B. "Molecular Typing of Cytotoxin-Producing Klebsiella Oxytoca Isolates by 16S-23S Internal Transcribed Spacer Pcr." New Microbes and New Infections 30, no. (2019): -.
Harvard	Soltan Dallal MM et al. (2019) 'Molecular Typing of Cytotoxin-Producing Klebsiella Oxytoca Isolates by 16S-23S Internal Transcribed Spacer Pcr', New Microbes and New Infections, 30(), pp. -.
Vancouver	Soltan Dallal MM, Validi M, Douraghi M, Bakhshi B. Molecular Typing of Cytotoxin-Producing Klebsiella Oxytoca Isolates by 16S-23S Internal Transcribed Spacer Pcr. New Microbes and New Infections. 2019;30():-.
BibTex	@article{ author = {Soltan Dallal MM and Validi M and Douraghi M and Bakhshi B}, title = {Molecular Typing of Cytotoxin-Producing Klebsiella Oxytoca Isolates by 16S-23S Internal Transcribed Spacer Pcr}, journal = {New Microbes and New Infections}, volume = {30}, number = {}, pages = {-}, year = {2019} }
RIS	TY - JOUR AU - Soltan Dallal MM AU - Validi M AU - Douraghi M AU - Bakhshi B TI - Molecular Typing of Cytotoxin-Producing Klebsiella Oxytoca Isolates by 16S-23S Internal Transcribed Spacer Pcr JO - New Microbes and New Infections VL - 30 IS - SP - EP - PY - 2019 ER -

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