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Genetic Diversity and Antibiotic Resistance Patterns of Escherichia Coli Isolates Causing Septicemia: A Phylogenetic Typing and Pfge Analysis Publisher Pubmed



Vakili M1, 2, 3 ; Goli H4 ; Javidnia J5 ; Alipour T6 ; Eslami M7, 8
Authors
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Authors Affiliations
  1. 1. Reference Laboratory of Health, Semnan University of Medical Sciences, Semnan, Iran
  2. 2. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Students' Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  5. 5. Invasive Fungi Research Centre (IFRC), Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran
  6. 6. Nervous System Stem Cell Research Center, Semnan University of Medical Sciences, Semnan, Iran
  7. 7. Department of Bacteriology and Virology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran
  8. 8. Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran

Source: Diagnostic Microbiology and Infectious Disease Published:2025


Abstract

Introduction: This study aims to analyze clinical isolates of E. coli causing septicemia across various phylogroups utilizing the PFGE method. Materials and methods: A total of 100 clinical isolates were collected. The presence of CTX-M, TEM, SHV, KPC, MBL and OXA-48 genes was detected by PCR. Additionally, phylotyping, serotyping, and virulence-typing assay were done by PCR and PFGE methods to investigate the genetic diversity of the isolates. Results: The O1 serotype and the HlyA gene were the most prevalent serotype and virulence gene, respectively. Notably, 34% of the isolates harbored SHV, TEM, and CTX-M-1 β-lactamase genes. All isolates showed resistance to amoxicillin and tetracycline, but no resistance to fosfomycin was seen. The most and least common phylotypes, according to PFGE analysis, belonged to phylogroups B2 and B1, respectively. Conclusion: The data offers valuable insights into the genetic diversity and antibiotic resistance patterns of E. coli isolates responsible for septicemia. © 2024
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