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Sandwich-Structured Nanoparticles-Grafted Functionalized Graphene Based 3D Nanocomposites for High-Performance Biosensors to Detect Ascorbic Acid Biomolecule Publisher Pubmed



Salahandish R1, 2, 3, 4 ; Ghaffarinejad A1, 2 ; Naghib SM5 ; Niyazi A5 ; Majidzadeha K6 ; Janmaleki M3, 4 ; Sanatinezhad A3, 4
Authors
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Authors Affiliations
  1. 1. Research Laboratory of Real Samples Analysis, Faculty of Chemistry, Iran University of Science and Technology (IUST), Tehran, 1684613114, Iran
  2. 2. Electroanalytical Chemistry Research Center, Iran University of Science and Technology (IUST), Tehran, 1684613114, Iran
  3. 3. BioMEMS and Bioinspired Microfluidic Laboratory, Department of Mechanical and Manufacturing Engineering, University of Calgary, Calgary, T2N 1N4, AB, Canada
  4. 4. Center for BioEngineering Research and Education, University of Calgary, Calgary, T2N 1N4, AB, Canada
  5. 5. Nanothechnology Department, School of New Technologies, Iran University of Science and Technology (IUST), P.O. Box 16846-13114, Tehran, Iran
  6. 6. Biomaterials and Tissue Engineering Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran

Source: Scientific Reports Published:2019


Abstract

We present a highly sensitive and selective nano-biosensor for rapid, stable and highly reproducible detection of ascorbic acid (AA) in the presence of dopamine, uric acid and other interferences by a three-layer sandwich arrangement of nitrogen-doped functionalized graphene (NFG), silver nanoparticles (AgNPs) and nanostructured polyaniline (PANI) nanocomposite. The enhanced AA electrochemical properties of the NFG/AgNPs/PANI electrode is attributed to the superior conductivity of the NFG-PANI and the excellent catalytic activity of AgNPs. The critical modification of the AgNPs-grafted NFG-PANI coated on very low-cost fluorine doped tin oxide electrode (FTOE) increased the charge transfer conductivity of the electrode (the resistance drops down from 11,000 Ω to 6 Ω). The nano-biosensor was used to accurately detect AA in vitamin C tablets with the recovery of 98%. The sensor demonstrated a low detection limit of 8 µM (S/N = 3) with a very wide linear detection range of 10–11,460 µM, good reproducibility and excellent selectivity performance for AA detection. The results demonstrate that this nanocomposite is a promising candidate for rapid and selective detection of AA in practical clinical samples. © 2019, The Author(s).