Anxiolytic-Like and Sedative Effects of Alcea Aucheri (Boiss.) Alef. Flower Extract in the Laboratory Rat



Mombeini T^{1, 2} ; Pourbadie HG^{3, 4} ; Kamalinejad M⁴ ; Mazloumi S⁵ ; Dehpour AR^{6, 7} Show Affiliations
Source: Iranian Journal of Pharmaceutical Research Published:2017

Abstract

The present study was conducted to investigate the possible anxiolytic and sedative of an acute administration and 4-day repeated dosing of an aqueous extract of flowers of Alcea aucheri (Boiss.) Alef. (EFA) in rats subjected to the elevated plus-maze (EPM), open-field, and horizontal wire tests. All drugs were administered intraperitoneally. Phytochemical screening confirmed the presence of phenolic compounds, flavonoids, and polysaccharides in the extract. Repeated dosing of EFA (at dose of 35 mg/kg) significantly increased percentage of time spent on open arms and of open arms entries, and also decreased percentage of time spent on closed arms and of closed arms entries; compared with saline control, 24 h after treatment. In addition, repeated dosing of EFA (at dose of 175 mg/kg) significantly increased open arm activity 48 h after treatment, versus saline group. This effect was also observed following acute administration of EFA at 175 mg/kg. In open field, acute administration of EFA at doses of 17.5, 35, 70, 175, 350, and 700 mg/kg induced a statistically significant and dose-dependent decrease in locomotor activity, compared with saline control. ED50 value for EFA-induced decrease in locomotor activity was 194 mg/kg. Furthermore, unlike diazepam; EFA didn´t decrease the percent of the rats grasping the wire. These data suggest that Alcea aucheri extract may have anxiolytic and sedative properties and some of the components in the extract such as phenolic compounds may have contributed to the observed effects. © 2017 by School of Pharmacy Shaheed Beheshti University of Medical Sciences and Health Services.