Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Clinical Grade Human Adipose Tissue-Derived Mesenchymal Stem Cell Banking Pubmed



Larijani B1, 2, 3, 4, 5, 6, 7, 8, 9, 10 ; Aghayan H1, 2, 3, 4, 5, 6, 7, 8, 9, 10 ; Goodarzi P1, 2, 3, 4, 5, 6, 7, 8, 9, 10 ; Mohamadijahani F1, 2, 3, 4, 5, 6, 7, 8, 9, 10 ; Norouzijavidan A1, 2, 3, 4, 5, 6, 7, 8, 9, 10 ; Dehpour AR1, 2, 3, 4, 5, 6, 7, 8, 9, 10 ; Fallahzadeh K1, 2, 3, 4, 5, 6, 7, 8, 9, 10 ; Azam Sayahpour F1, 2, 3, 4, 5, 6, 7, 8, 9, 10 ; Bidaki K1, 2, 3, 4, 5, 6, 7, 8, 9, 10 ; Arjmand B1, 2, 3, 4, 5, 6, 7, 8, 9, 10
Authors
Show Affiliations
Authors Affiliations
  1. 1. Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  8. 8. Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  9. 9. Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  10. 10. Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Acta medica Iranica Published:2015


Abstract

In this study, our aim was to produce a generation of GMP-grade adipose tissue-derived mesenchymal stem cells for clinical applications. According to our results, we fulfill to establish consistent and also reproducible current good manufacturing practice (cGMP) compliant adipose tissue-derived mesenchymal stem cells from five female donors. The isolated cells were cultured in DMEM supplemented with 10% fetal bovine serum and characterized by standard methods. Moreover, karyotyping was performed to evaluate chromosomal stability. Mean of donors' age was 47.6 ± 8.29 year, mean of cell viability was 95.6 ± 1.51%, and cell count was between 9×106 and 14×106 per microliter with the mean of 12.2×106 ± 2863564.21 per microliter. The main aim of this project was demonstrating the feasibility of cGMP-compliant and clinical grade adipose tissue-derived mesenchymal stem cells preparation and banking for clinical cell transplantation trials.