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First Molecular Report of Hydatigera Krepkogorski (Schulz and Landa, 1934) in Abdominal Cavity of Gerbil (Rhombomys Opimus) in Iran Publisher



Kia EB1 ; Sharbatkhori M2, 3 ; Heidari Z4 ; Tohidi F5 ; Rashani BK1 ; Zahabiun F1
Authors
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Authors Affiliations
  1. 1. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran
  3. 3. Department of Medical Parasitology and Mycology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
  4. 4. Department of Medical Microbiology and Parasitology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
  5. 5. Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran

Source: Veterinary Research Forum Published:2018


Abstract

A polycephalic larva of Taeniidae family isolated from abdominal cavity of a great gerbil (Rhombomys opimus) from Golestan province, northern Iran, was subjected to molecular analysis. Genomic DNA from the larva was obtained using a DNA extraction tissue kit. Polymerase chain reaction was performed for amplification of the partial 12S rRNA, cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase 1 (nad1) mitochondrial genes. BLAST analysis of DNA sequencing indicated 99.00% homology in 12S rRNA and cox1 genes and 98.00% homology in nad1 gene with Hydatigera krepkogorski (accession No. AB731762). The sequences of current isolate were deposited in GenBank by accession Nos. MF281971, MF281972 and MF281973 for 12 SrRNA, cox1 and nad1 genes, respectively. This study was the first report of molecular characterization of H. krepkogorski from Iran. Isolation and characterization of the adult stage from definitive host will help to better clarify incomplete life cycle and morphology data of this species in the world. © 2018 Urmia University. All rights reserved.
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