Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Molecular Characterization of Fasciola Spp. From Some Parts of Iran



Hasanpour H1, 2 ; Falak R3 ; Naddaf SR4 ; Mascoma S5 ; Rokni MB1 ; Badirzadeh A6 ; Mokhtarian K7 ; Mohebali M1 ; Jafarpour Azami S1 ; Fadavi A1 ; Gharagozlou MJ8 ; Mohammad K1 ; Mowlavi G1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Parasitology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran
  3. 3. Immunology Research Center, Iran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran
  5. 5. Department of Parasitology, Faculty of Pharmacy, University of Valencia, Valencia, Spain
  6. 6. Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Parasitology and Mycology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran
  8. 8. Department of Pathobiology, School of Veterinary Medicine, University of Tehran, Tehran, Iran

Source: Iranian Journal of Public Health Published:2020

Abstract

Background: Identification of liver flukes, Fasciola hepatica, and Fasciola gigantica by morphometric parameters is not always reliable due to the overlapping measurements. This study aimed to characterize the liver flukes of animals from different parts of Iran by the genetic markers, ITS1, and COXI. Methods: We collected flukes from infected livestock in six provinces of Iran from Sep to Nov 2016. The flukes were identified by amplification of a 680 bp sequence of ITS1 locus followed by a restriction fragment polymor-phism (RFLP) assay. The genetic diversity among isolates was evaluated by amplification and sequencing of a 493 bp fragment of the COXI gene. Results: We obtained 38 specimens from Khuzestan, 22 from Tehran, 10 from Isfahan, 10 from Mazandaran, 4 from Kurdistan, and 3 from Ardabil provinces. PCR-RFLP analysis revealed two patterns, representing F. hepatica, and F. gigantica. Fifty specimens from cattle and sheep exhibited F. hepatica pattern and 37 from the cattle, sheep, buffalo, and goat that of F. gigantica. The phylogeny based on COXI revealed two distinct clades separating F. hepatica from F. gigantica. In our phylogeny, the Iranian F. gigantica isolates showed a distinct separation from the African flukes, while grouped with the East Asia specimens demonstrating a common ancestor. The F. hepatica isolates clustered with the flukes from different parts of the world, including East Asia, Europe, and South America. Conclusion: The present study revealed a substantial genetic difference between F. gigantica populations of Asia and Africa, while F. hepatica isolates from different parts of the world shared high similarities. © 2020, Iranian Journal of Public Health. All rights reserved.
Related Docs
View other Related Docs
1. Molecular Characterization of Animal Fasciola Spp. Isolates From Lorestan Province, Western Iran, Iranian Journal of Public Health (2022)
2. Morphological and Molecular Characterization of Fasciola Isolates From Livestock in Golestan Province, Northern Iran, Veterinary Medicine and Science (2023)
3. Identification and Differentiation of Fasciola Hepatica and F. Gigantica Using Multiplex Pcr Technique, Annals of Parasitology (2023)
4. Molecular and Morphometrical Characterization of Fasciola Species Isolated From Domestic Ruminants in Ardabil Province, Northwestern Iran, Iranian Journal of Public Health (2017)
5. Molecular Characterization of Animal Fasciola Spp. Isolates From Kermanshah,Western Iran, Iranian Journal of Public Health (2016)
6. Specific Detection of Fasciola Hepatica and F. Gigantica in Infected Domesticated Animals Using High-Resolution Melting Analysis (Hrm), Iranian Journal of Public Health (2020)
7. Prevalence of Fasciolosis in Livestock and Humans: A Systematic Review and Meta-Analysis in Iran, Comparative Immunology# Microbiology and Infectious Diseases (2019)
8. Molecular Phylogenetic and Genetic Variability of Fasciola Gigantica in Kermanshah Province, Western Iran With an Overview to Understand Haplotypes Distribution in Asia and Africa, Veterinary Research Forum (2020)
Experts (# of related papers)
View all Related Experts
Mahdi Mohebali (7)
Eshrat Beigom Kia (5)
Other Related Docs
9. Seroprevalence of Human Fasciolosis in Lorestan Province, Western Iran, in 2015-16, Iranian Journal of Parasitology (2017)
10. Emerging Cases of Fascioliasis in Lorestan Province, Western Iran: Case Series Report, Iranian Journal of Public Health (2021)
11. Ecological Niche Modeling for Predicting the Habitat Suitability of Fascioliasis Based on Maximum Entropy Model in Southern Caspian Sea Littoral, Iran, Acta Tropica (2019)
12. Identification of Fasciola Species Using Tegumental Spines in Tissue Sections, Iranian Journal of Public Health (2020)
13. Seroepidemiology of Human Fascioliasis in Rural and Nomad Areas of Lorestan Province, Western Iran, in 2016 and 2017, Journal of Parasitic Diseases (2020)
14. High-Resolution Melting Analysis As an Appropriate Method to Differentiate Between Fasciola Hepatica and F. Gigantica, Iranian Journal of Public Health (2019)
15. Bioclimatic Analysis and Spatial Distribution of Livestock Fascioliasis in Iran, Iranian Journal of Parasitology (2019)
16. Identification and Phylogenetic Classification of Fasciola Species Isolated From Sheep and Cattle by Pcr-Rflp in Zabol, in Sistan and Baluchistan Province, Southeast Iran, Iranian Journal of Public Health (2019)
17. Seroprevalence of Human Fasciolosis in Pirabad, Lorestan Province, Western Iran, Iranian Journal of Parasitology (2016)
18. The Endemicity of Human Fascioliasis in Guilan Province, Northern Iran: The Baseline for Implementation of Control Strategies, Iranian Journal of Public Health (2015)
19. Evaluation of Semi-Nested Pcr Compared With Indirect-Elisa to Diagnose Human Fasciolosis, Iranian Journal of Public Health (2022)
20. Molecular Evidence of Human Fasciolosis Due to Fasciola Gigantica in Iran: A Case Report, Iranian Journal of Public Health (2018)
21. Spatial Modeling and Risk Mapping of Human Fascioliasis in Iran: A Geographical Information System-Based Survey From 2013-2022, Archives of Clinical Infectious Diseases (2024)
22. Detection Cell-Free Dna (Cfdna) Using Nested-Pcr As a Diagnosis Tool for Human Fascioliasis Infection, Iranian Journal of Public Health (2020)
23. Assessment of Genetic Markers for Multilocus Sequence Typing (Mlst) of Fasciola Isolates From Iran, Veterinary Medicine and Science (2023)
24. Emerging and Re-Emerging Infectious Diseases in Iran, Iranian Journal of Microbiology (2017)
25. Epidemiology of Fascioliasis in Kermanshah Province, Western Iran, Iranian Journal of Public Health (2018)
26. Molecular Analysis of Internal Transcribed Spacer 2 of Dicrocoelium Dendriticum Isolated From Cattle, Sheep, and Goat in Iran, BMC Veterinary Research (2022)
27. Fasciola Infection Unexpectedly Found During Cholecystectomy: Review on How to Avoid Increasing Surgery Interventions in Non-Human Endemic Areas, Acta Parasitologica (2023)
28. Serological Study of Human Fasciolosis in Patients Referring to the School of Public Health, Tehran University of Medical Sciences, Tehran, Iran During 2008-2014, Iranian Journal of Parasitology (2015)