Fatty Acid Synthase and Acetyl-Coa Carboxylase Dysfunction in Visceral Adipose Tissue



Khalili E¹ ; Pour NJA² ; Meshkani R¹ ; Toulabi K³ ; Emamgholipour S¹ Show Affiliations
Source: Journal of Payavard Salamat Published:2020

Abstract

Background and Aim: A great interest in determining the particular mechanisms underlying lipogenesis and adipogenesis has been raised among researchers to fight obesity. We aimed to investigate the gene expression of fatty acid synthase(FAS) and Acetyl-CoA carboxylase(ACC) and its role in the regulation of lipogenesis and adipogenesis in visceral adipose tissues from obese and normal-weight subjects. Materials and Methods: A total of 39 participants including 19 obese patients(body mass index≥35 kg/m2) and 20 healthy subjects(body mass index between 18.5 to 24.9 kg/m2) were recruited from who were referred to Erfan, Loghman Hakim, Sina, and Imam Khomeini hospitals bypass or sleeve gastrectomy surgeries in obese ones and elective surgery in controls. Participants were all women aged from 20-50 years and postmenopausal subjects were not included in this study. Isolated total RNA from visceral adipose tissue(VAT) was used to synthesize complementary DNA(cDNA) and quantitative real-time PCR was performed for analyzing the gene expressions of FAS, and ACC. Data were normalized to geometric means of GAPDH and β-actin expression levels. Results: In VAT from obese subjects, gene expression of FAS was higher than in those from controls. We found a positive correlation between gene expression of FAS and ACC with obesity indices including BMI, waist circumference, and hip circumference. Conclusion: It appears that obesity is associated with dysregulation of FAS genes involved in lipogenesis and adipogenesis. © 2020, Tehran University of Medical Sciences. All rights reserved.