Involvement of Nrf2 in Development of Anxiety-Like Behavior by Linking Bcl2 to Oxidative Phosphorylation: Estimation in Rat Hippocampus, Amygdala, and Prefrontal Cortex

Involvement of Nrf2 in Development of Anxiety-Like Behavior by Linking Bcl2 to Oxidative Phosphorylation: Estimation in Rat Hippocampus, Amygdala, and Prefrontal Cortex Publisher Pubmed

Khalifeh S¹ ; Oryan S¹ ; Digaleh H^{2, 3} ; Shaerzadeh F⁴ ; Khodagholi F^{2, 3} ; Maghsoudi N^{2, 3} ; Zarrindast MR⁵

Show Affiliations

1. Department of Animal Physiology, Kharazmi (Tarbiat Moallem) University, Tehran, Iran
2. NeuroBiology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3. Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4. Neurophysiology Research Center, Department of Physiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Molecular Neuroscience Published:2015

Abstract

Anxiety-related disorders are complex illnesses that underlying molecular mechanisms of these complicated emotional disorders are poorly understood. Nuclear factor erythroid 2-related factor 2 (Nrf2) is the most important regulator of the antioxidant defense system. Its protective actions are not only limited to antioxidative transactivation, but also plays important roles in encountering various physiological and pathological stresses. In this study, we evaluated whether silencing of Nrf2 plays a role in development of anxiety-related behavior. In this regard, we exerted small interfering RNA (siRNA) targeting Nrf2 in dorsal third ventricle and subsequently examined the effect of this silencing on anxiety-related behavior along with supposed molecular mechanisms. Therefore, we evaluated apoptotic markers and mitochondrial electron transport chain (ETC) activity in three brain regions: hippocampus, amygdala, and prefrontal cortex. Based on our result, Nrf2-silenced rats exhibited greater anxiety-like behavior compared to control group. Furthermore, Nrf2 silencing increased activity of ETC complexes. Also, Bax/Bcl2 ratio of all mentioned areas of the brain and cleavage of caspase-3 in hippocampus increased in Nrf2 silenced group, however, with a distinct pattern. © 2014, Springer Science+Business Media New York.

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Style	Citing Format
MLA	Khalifeh S, et al.. "Involvement of Nrf2 in Development of Anxiety-Like Behavior by Linking Bcl2 to Oxidative Phosphorylation: Estimation in Rat Hippocampus, Amygdala, and Prefrontal Cortex." Journal of Molecular Neuroscience, vol. 55, no. 2, 2015, pp. 492-499.
APA	Khalifeh S, Oryan S, Digaleh H, Shaerzadeh F, Khodagholi F, Maghsoudi N, Zarrindast MR (2015). Involvement of Nrf2 in Development of Anxiety-Like Behavior by Linking Bcl2 to Oxidative Phosphorylation: Estimation in Rat Hippocampus, Amygdala, and Prefrontal Cortex. Journal of Molecular Neuroscience, 55(2), 492-499.
Chicago	Khalifeh S, Oryan S, Digaleh H, Shaerzadeh F, Khodagholi F, Maghsoudi N, Zarrindast MR. "Involvement of Nrf2 in Development of Anxiety-Like Behavior by Linking Bcl2 to Oxidative Phosphorylation: Estimation in Rat Hippocampus, Amygdala, and Prefrontal Cortex." Journal of Molecular Neuroscience 55, no. 2 (2015): 492-499.
Harvard	Khalifeh S et al. (2015) 'Involvement of Nrf2 in Development of Anxiety-Like Behavior by Linking Bcl2 to Oxidative Phosphorylation: Estimation in Rat Hippocampus, Amygdala, and Prefrontal Cortex', Journal of Molecular Neuroscience, 55(2), pp. 492-499.
Vancouver	Khalifeh S, Oryan S, Digaleh H, Shaerzadeh F, Khodagholi F, Maghsoudi N, et al.. Involvement of Nrf2 in Development of Anxiety-Like Behavior by Linking Bcl2 to Oxidative Phosphorylation: Estimation in Rat Hippocampus, Amygdala, and Prefrontal Cortex. Journal of Molecular Neuroscience. 2015;55(2):492-499.
BibTex	@article{ author = {Khalifeh S and Oryan S and Digaleh H and Shaerzadeh F and Khodagholi F and Maghsoudi N and Zarrindast MR}, title = {Involvement of Nrf2 in Development of Anxiety-Like Behavior by Linking Bcl2 to Oxidative Phosphorylation: Estimation in Rat Hippocampus, Amygdala, and Prefrontal Cortex}, journal = {Journal of Molecular Neuroscience}, volume = {55}, number = {2}, pages = {492-499}, year = {2015} }
RIS	TY - JOUR AU - Khalifeh S AU - Oryan S AU - Digaleh H AU - Shaerzadeh F AU - Khodagholi F AU - Maghsoudi N AU - Zarrindast MR TI - Involvement of Nrf2 in Development of Anxiety-Like Behavior by Linking Bcl2 to Oxidative Phosphorylation: Estimation in Rat Hippocampus, Amygdala, and Prefrontal Cortex JO - Journal of Molecular Neuroscience VL - 55 IS - 2 SP - 492 EP - 499 PY - 2015 ER -

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