Inter- and Intra-Subtype Variation of Blastocystis Subtypes Isolated From Diarrheic and Non-Diarrheic Patients in Iran

Inter- and Intra-Subtype Variation of Blastocystis Subtypes Isolated From Diarrheic and Non-Diarrheic Patients in Iran Publisher Pubmed

Alinaghizade A¹ ; Mirjalali H² ; Mohebali M¹ ; Stensvold CR³ ; Rezaeian M¹

Show Affiliations

1. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2. Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3. Laboratory of Parasitology, Department of Bacteria, Parasites & Fungi, Statens Serum Institut, Copenhagen, Denmark

Source: Infection# Genetics and Evolution Published:2017

Abstract

Blastocystis is a common intestinal parasitic protist infecting birds and mammals. Blastocystis comprises at least 17 subtypes (ST), of which ST1–ST9 have been detected in humans. Significant correlation between certain subtypes and pathogenicity remains to be established. Nevertheless, some studies suggest a potential linkage between subtypes (inter- and intra-subtype variation) and clinical manifestations. The aim of this study was to identify intra-subtype genetic variation of subtypes of Blastocystis in stools samples submitted by diarrheic and non-diarrheic patients. A 550-bp fragment of the nuclear small subunit ribosomal rRNA gene was amplified from 58 culture-positive samples isolated from diarrheic and non-diarrheic Iranian patients. PCR products were sequenced and sequences subjected to phylogenetic analysis. Intra-and inter-subtype variation was calculated. Based on comparison with reference sequences in GenBank, ST1, ST2 and ST3 were found in 18 (31.03%), 21 (36.22%), and 19 (32.75%) of the samples, respectively. Diarrheic stools were observed in eight (44.44%), 10 (47.61%), and nine (47.36%) patients with ST1, ST2, and ST3, respectively. No statistically significant correlation was found between subtypes and diarrhea (P = 1.000). Multiple sequence alignment exhibited a within-subtype similarity of 98.76%, 97.17%, and 99.78% in ST1, ST2, and ST3, respectively. Highest similarity was seen among ST3 isolates, while lowest similarity was seen among ST2 isolates. Phylogenetic analysis did not suggest any correlation between diarrhea and intra-subtype variation. Inter- and intra-subtype variation in SSU rRNA gene appears not to reflect differences in the clinical outcome of Blastocystis carriage. © 2017 Elsevier B.V.

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Style	Citing Format
MLA	Alinaghizade A, et al.. "Inter- and Intra-Subtype Variation of Blastocystis Subtypes Isolated From Diarrheic and Non-Diarrheic Patients in Iran." Infection# Genetics and Evolution, vol. 50, no. , 2017, pp. 77-82.
APA	Alinaghizade A, Mirjalali H, Mohebali M, Stensvold CR, Rezaeian M (2017). Inter- and Intra-Subtype Variation of Blastocystis Subtypes Isolated From Diarrheic and Non-Diarrheic Patients in Iran. Infection# Genetics and Evolution, 50(), 77-82.
Chicago	Alinaghizade A, Mirjalali H, Mohebali M, Stensvold CR, Rezaeian M. "Inter- and Intra-Subtype Variation of Blastocystis Subtypes Isolated From Diarrheic and Non-Diarrheic Patients in Iran." Infection# Genetics and Evolution 50, no. (2017): 77-82.
Harvard	Alinaghizade A et al. (2017) 'Inter- and Intra-Subtype Variation of Blastocystis Subtypes Isolated From Diarrheic and Non-Diarrheic Patients in Iran', Infection# Genetics and Evolution, 50(), pp. 77-82.
Vancouver	Alinaghizade A, Mirjalali H, Mohebali M, Stensvold CR, Rezaeian M. Inter- and Intra-Subtype Variation of Blastocystis Subtypes Isolated From Diarrheic and Non-Diarrheic Patients in Iran. Infection# Genetics and Evolution. 2017;50():77-82.
BibTex	@article{ author = {Alinaghizade A and Mirjalali H and Mohebali M and Stensvold CR and Rezaeian M}, title = {Inter- and Intra-Subtype Variation of Blastocystis Subtypes Isolated From Diarrheic and Non-Diarrheic Patients in Iran}, journal = {Infection# Genetics and Evolution}, volume = {50}, number = {}, pages = {77-82}, year = {2017} }
RIS	TY - JOUR AU - Alinaghizade A AU - Mirjalali H AU - Mohebali M AU - Stensvold CR AU - Rezaeian M TI - Inter- and Intra-Subtype Variation of Blastocystis Subtypes Isolated From Diarrheic and Non-Diarrheic Patients in Iran JO - Infection# Genetics and Evolution VL - 50 IS - SP - 77 EP - 82 PY - 2017 ER -

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