Monoclonal Antibody Against Sortilin Induces Apoptosis in Human Breast Cancer Cells

Monoclonal Antibody Against Sortilin Induces Apoptosis in Human Breast Cancer Cells Publisher

Simonian M¹ ; Ghaffari MH¹ ; Salimi A² ; Mirzadegan E³ ; Sadeghi N² ; Ebrahimnezhad N² ; Fazli G² ; Fatemi R² ; Bayat AA² ; Milani S⁴ ; Negahdari B¹ ; Rabbani H²

Show Affiliations

1. Department of edical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
3. Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
4. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran

Source: Avicenna Journal of Medical Biotechnology Published:2022

Abstract

Background: Sortilin has an important role in various malignances and can be used as a promising target to eradicate cancer cells. Methods: In this study, the expression of sortilin in 4T1 and MDA-MB231 cell lines was evaluated by flow cytometry and immunocytochemistry. Apoptosis assay was also applied to evaluate apoptosis induction in 4T1 and MDA-MB231 cell lines. Results: Based on cell surface flow cytometry results, anti-sortilin (2D8-E3) mAb could recognize sortilin molecules in 79.2% and 90.3% of 4T1 and MDA-MB231 cell-lines, respectively. The immunocytochemistry staining results confirmed sortilin surface expression. Apoptosis assay indicated that anti-sortilin mAb could induce apoptosis in 4T1 and MDA-MB231 cell lines. Conclusion: Our study revealed the important role of surface sortilin in breast carcinoma cell survival and its possible application as a therapeutic agent in cancer targeted therapies. © 2022, Avicenna Journal of Medical Biotechnology. All rights reserved.

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Style	Citing Format
MLA	Simonian M, et al.. "Monoclonal Antibody Against Sortilin Induces Apoptosis in Human Breast Cancer Cells." Avicenna Journal of Medical Biotechnology, vol. 14, no. 1, 2022, pp. 37-45.
APA	Simonian M, Ghaffari MH, Salimi A, Mirzadegan E, Sadeghi N, Ebrahimnezhad N, Fazli G, Fatemi R, Bayat AA, Milani S, Negahdari B, Rabbani H (2022). Monoclonal Antibody Against Sortilin Induces Apoptosis in Human Breast Cancer Cells. Avicenna Journal of Medical Biotechnology, 14(1), 37-45.
Chicago	Simonian M, Ghaffari MH, Salimi A, Mirzadegan E, Sadeghi N, Ebrahimnezhad N, Fazli G, et al.. "Monoclonal Antibody Against Sortilin Induces Apoptosis in Human Breast Cancer Cells." Avicenna Journal of Medical Biotechnology 14, no. 1 (2022): 37-45.
Harvard	Simonian M et al. (2022) 'Monoclonal Antibody Against Sortilin Induces Apoptosis in Human Breast Cancer Cells', Avicenna Journal of Medical Biotechnology, 14(1), pp. 37-45.
Vancouver	Simonian M, Ghaffari MH, Salimi A, Mirzadegan E, Sadeghi N, Ebrahimnezhad N, et al.. Monoclonal Antibody Against Sortilin Induces Apoptosis in Human Breast Cancer Cells. Avicenna Journal of Medical Biotechnology. 2022;14(1):37-45.
BibTex	@article{ author = {Simonian M and Ghaffari MH and Salimi A and Mirzadegan E and Sadeghi N and Ebrahimnezhad N and Fazli G and Fatemi R and Bayat AA and Milani S and Negahdari B and Rabbani H}, title = {Monoclonal Antibody Against Sortilin Induces Apoptosis in Human Breast Cancer Cells}, journal = {Avicenna Journal of Medical Biotechnology}, volume = {14}, number = {1}, pages = {37-45}, year = {2022} }
RIS	TY - JOUR AU - Simonian M AU - Ghaffari MH AU - Salimi A AU - Mirzadegan E AU - Sadeghi N AU - Ebrahimnezhad N AU - Fazli G AU - Fatemi R AU - Bayat AA AU - Milani S AU - Negahdari B AU - Rabbani H TI - Monoclonal Antibody Against Sortilin Induces Apoptosis in Human Breast Cancer Cells JO - Avicenna Journal of Medical Biotechnology VL - 14 IS - 1 SP - 37 EP - 45 PY - 2022 ER -

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