Inhibitory Effect of Flavonoid Xanthomicrol on Triple-Negative Breast Tumor Via Regulation of Cancer-Associated Micrornas

Inhibitory Effect of Flavonoid Xanthomicrol on Triple-Negative Breast Tumor Via Regulation of Cancer-Associated Micrornas Publisher Pubmed

Attari F¹ ; Keighobadi F² ; Abdollahi M³ ; Arefian E⁴ ; Lotfizadeh R¹ ; Sepehri H¹ ; Moridi Farimani M⁵

Show Affiliations

1. Department of Animal Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran
2. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
3. Toxicology and Diseases Group, Pharmaceutical Sciences Research Center (PSRC), The Institute of Pharmaceutical Sciences (TIPS), and Department of Toxicology and Pharmacology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
4. Molecular Virology Lab, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
5. Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran

Source: Phytotherapy Research Published:2021

Abstract

Breast cancer is the leading cause of cancer death in women worldwide. Due to the side effects of current chemo-reagents on healthy tissues, it is essential to search for alternative compounds with less toxicity and better efficacy. In the present study, we have investigated the anticancer effects of flavonoid xanthomicrol on the mice breast cancer model using MTT assay, cell cycle and Annexin/PI analysis, colony formation assay, H&E staining, immunohistochemistry, and miRNA analysis. Our results demonstrated that xanthomicrol decreased the cell viability and clonogenic capability, induced G1-arrest and apoptosis in the breast cancer cells in vitro, and caused a significant reduction in the volume and weight of mice tumors in vivo. In addition, xanthomicrol reduced the expression of TNFα, VEGF, MMP9, and Ki67, while upregulating the expression of apoptotic markers such as Bax, caspase3, and caspase9. Finally, the expression of miR21, miR27, and miR125, known as oncomirs, decreased significantly after xanthomicrol administration, while the expression of miR29 and miR34, functioning as tumor suppressors, increased significantly (p <.001). Our data demonstrated that xanthomicrol can induce apoptosis and suppress angiogenesis in breast cancer cells due to its inhibitory effect on oncomirs and its stimulatory effect on tumor suppressor miRNAs. © 2020 John Wiley & Sons Ltd

2. Flavonoids Target Different Molecules of Autophagic and Metastatic Pathways in Cancer Cells, Cancer Cell International (2023)

Style	Citing Format
MLA	Attari F, et al.. "Inhibitory Effect of Flavonoid Xanthomicrol on Triple-Negative Breast Tumor Via Regulation of Cancer-Associated Micrornas." Phytotherapy Research, vol. 35, no. 4, 2021, pp. 1967-1982.
APA	Attari F, Keighobadi F, Abdollahi M, Arefian E, Lotfizadeh R, Sepehri H, Moridi Farimani M (2021). Inhibitory Effect of Flavonoid Xanthomicrol on Triple-Negative Breast Tumor Via Regulation of Cancer-Associated Micrornas. Phytotherapy Research, 35(4), 1967-1982.
Chicago	Attari F, Keighobadi F, Abdollahi M, Arefian E, Lotfizadeh R, Sepehri H, Moridi Farimani M. "Inhibitory Effect of Flavonoid Xanthomicrol on Triple-Negative Breast Tumor Via Regulation of Cancer-Associated Micrornas." Phytotherapy Research 35, no. 4 (2021): 1967-1982.
Harvard	Attari F et al. (2021) 'Inhibitory Effect of Flavonoid Xanthomicrol on Triple-Negative Breast Tumor Via Regulation of Cancer-Associated Micrornas', Phytotherapy Research, 35(4), pp. 1967-1982.
Vancouver	Attari F, Keighobadi F, Abdollahi M, Arefian E, Lotfizadeh R, Sepehri H, et al.. Inhibitory Effect of Flavonoid Xanthomicrol on Triple-Negative Breast Tumor Via Regulation of Cancer-Associated Micrornas. Phytotherapy Research. 2021;35(4):1967-1982.
BibTex	@article{ author = {Attari F and Keighobadi F and Abdollahi M and Arefian E and Lotfizadeh R and Sepehri H and Moridi Farimani M}, title = {Inhibitory Effect of Flavonoid Xanthomicrol on Triple-Negative Breast Tumor Via Regulation of Cancer-Associated Micrornas}, journal = {Phytotherapy Research}, volume = {35}, number = {4}, pages = {1967-1982}, year = {2021} }
RIS	TY - JOUR AU - Attari F AU - Keighobadi F AU - Abdollahi M AU - Arefian E AU - Lotfizadeh R AU - Sepehri H AU - Moridi Farimani M TI - Inhibitory Effect of Flavonoid Xanthomicrol on Triple-Negative Breast Tumor Via Regulation of Cancer-Associated Micrornas JO - Phytotherapy Research VL - 35 IS - 4 SP - 1967 EP - 1982 PY - 2021 ER -

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