Evaluation of Sperm Dna Fragmentation and Chromatin Structure in Infertile Men With Immotile Short-Tail Sperm Defect

Evaluation of Sperm Dna Fragmentation and Chromatin Structure in Infertile Men With Immotile Short-Tail Sperm Defect Publisher Pubmed

Atshan M¹ ; Kakavand K¹ ; Hosseini SH¹ ; Sadighi Gilani MA^{1, 2} ; Mohseni Meybodi A³ ; Sabbaghian M¹

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1. Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
2. Department of Urology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

Source: Andrologia Published:2020

Abstract

Teratozoospermia is characterised by the presence of spermatozoa with abnormal morphology. One of the morphological disorders that lead to male infertility is immotile short-tail sperm (ISTS) defect. In this study, we evaluated the levels of chromatin packing and DNA fragmentation in patients with immotile short-tail sperm defect. Semen samples were obtained from 31 infertile men with ISTS as case group and 31 normozoospermic men as a control group. Protamine status was evaluated using chromomycin A3 (CMA3) staining and sperm DNA fragmentation assessed by sperm chromatin structure assay (SCSA) and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick-end labelling (TUNEL). The percentage of positive CMA3 spermatozoa was significantly higher in patients’ samples (22.6 ± 6.9) compared with controls (16.3 ± 4.2) (p <.05) and also mean (±SD) of sperm DNA fragmentation was significantly higher in patients compared with controls, as measured by TUNEL assay (10.45 ± 4.60 vs. 7.03 ± 2.86, p <.05) and SCSA (24.80 ± 13.1 vs. 15.2 ± 7.2, p <.05). According to our study, sperm DNA fragmentation and chromatin packing abnormality are significantly higher in the ISTS samples compared with normal samples. A possible explanation for this relationship is that sperm chromatin condensation and sperm flagellum formation occur during the same phase of spermatogenesis. © 2019 Blackwell Verlag GmbH

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Mohammad Ali Sadighi Gilani (5)

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Style	Citing Format
MLA	Atshan M, et al.. "Evaluation of Sperm Dna Fragmentation and Chromatin Structure in Infertile Men With Immotile Short-Tail Sperm Defect." Andrologia, vol. 52, no. 1, 2020, pp. -.
APA	Atshan M, Kakavand K, Hosseini SH, Sadighi Gilani MA, Mohseni Meybodi A, Sabbaghian M (2020). Evaluation of Sperm Dna Fragmentation and Chromatin Structure in Infertile Men With Immotile Short-Tail Sperm Defect. Andrologia, 52(1), -.
Chicago	Atshan M, Kakavand K, Hosseini SH, Sadighi Gilani MA, Mohseni Meybodi A, Sabbaghian M. "Evaluation of Sperm Dna Fragmentation and Chromatin Structure in Infertile Men With Immotile Short-Tail Sperm Defect." Andrologia 52, no. 1 (2020): -.
Harvard	Atshan M et al. (2020) 'Evaluation of Sperm Dna Fragmentation and Chromatin Structure in Infertile Men With Immotile Short-Tail Sperm Defect', Andrologia, 52(1), pp. -.
Vancouver	Atshan M, Kakavand K, Hosseini SH, Sadighi Gilani MA, Mohseni Meybodi A, Sabbaghian M. Evaluation of Sperm Dna Fragmentation and Chromatin Structure in Infertile Men With Immotile Short-Tail Sperm Defect. Andrologia. 2020;52(1):-.
BibTex	@article{ author = {Atshan M and Kakavand K and Hosseini SH and Sadighi Gilani MA and Mohseni Meybodi A and Sabbaghian M}, title = {Evaluation of Sperm Dna Fragmentation and Chromatin Structure in Infertile Men With Immotile Short-Tail Sperm Defect}, journal = {Andrologia}, volume = {52}, number = {1}, pages = {-}, year = {2020} }
RIS	TY - JOUR AU - Atshan M AU - Kakavand K AU - Hosseini SH AU - Sadighi Gilani MA AU - Mohseni Meybodi A AU - Sabbaghian M TI - Evaluation of Sperm Dna Fragmentation and Chromatin Structure in Infertile Men With Immotile Short-Tail Sperm Defect JO - Andrologia VL - 52 IS - 1 SP - EP - PY - 2020 ER -

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