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In Vitro Activity of Two Nanoparticles on Clinical Isolates of Candida Parapsilosis, Showing Resistance Against Antifungal Agents in Children Publisher



Lotfali E1 ; Shahverdi AR2 ; Mohammadi R3 ; Noorbakhsh F4 ; Ghajari A1 ; Ansari S1 ; Rezaie S5
Authors
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Authors Affiliations
  1. 1. Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Department of Pharmaceutical Biotechnology and Biotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Medical Parasitology and Mycology, School of Medicine, Infectious Disease and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Microbiology, Biological Science College, Varamin-Pishva Branch, Islamic Azad University, Varamin-Pishva, Iran
  5. 5. Division of Molecular Biology, Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: Archives of Clinical Infectious Diseases Published:2017


Abstract

Background: Resistance of pathogenic fungi to conventionally available antifungal agents has been increasing and has become a serious problem. On the other hand, nanoscience has emerged as a powerful tool capable of developing and designing new antimicrobial drugs. Objectives: The aim of this study was to test minimum inhibitory concentrations (MICs) of silver (Ag-NPs) and selenium (Se-NPs) nanoparticles, both alone and in combination with antifungal agents fluconazole (FLU), itraconazole (ITR), and amphotericin B (AMB) on resistant C. parapsilosis strains. Methods: Eight C. parapsilosis strains resistant to antifungal agents (FLU, ITR, AMB) and the standard strain of C. parapsilosis (ATCC 22019) as a control were used in this study. The standard protocol of antifungal susceptibility testing for yeast was performed by the broth micro-dilution method described in the clinical and laboratory standards institute (CLSI) guidelines, documentM27-S4. Results and Conclusions: MICs of standard and resistant strains were different against nano compounds, both alone and in combination with antifungal drugs. The growth of yeast was inhibited by Ag-NPs at concentrations as low as 2 µg/mL (against FLCR1 and FLCR3) and 0.25 µg /mL (against ITRR2 and AMBR2). Antifungal activity testing of Se-NPs revealed that it can inhibit the growth of yeasts at concentrations as low as 4 µg /mL (against FLCR1 and FLCR3) and 0.5 mg/mL (against ITRR2, ITRR3 and AMBR1). The results demonstrated that Se-NPs had lower activity than Ag-NPs, but both (Se-NPs and Ag-NPs) had higher activity thancommonantifungal agents. Further in vitro and in vivo studies are required for this. © 2017, Archives of Clinical Infectious Diseases.
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