Does in Vitro Fertilization Affect the Expression of Mirnas and Their Biogenesis Pathway in Preimplantation Mouse Embryos? Publisher Pubmed



Azizi E¹ ; Ghaffari Novin M^{1, 2} ; Naji M³ ; Amidi F⁴ ; Shams Mofarahe Z¹ Show Affiliations
Source: Birth Defects Research Published:2020

Abstract

Background: In vitro fertilization (IVF) is a well-accepted procedure which has been utilized for the treatment of infertile patients. As embryos at early stages of development are very vulnerable, the IVF conditions may influence genetic and epigenetic regulation of preimplantation mouse embryo. Methods: We assessed the effect of IVF on the expression of developmental and implantation related miRNAs (miR-21, miR-93, miR-24, and let-7a), their common presumptive target (Stat3), and miRNA biogenesis pathway genes (Drosha, Dgcr8, Exportin-5, Dicer, and Ago2). in vivo 8-cell and blastocysts were compared to IVF embryos. Expression levels of miRNAs, Stat3, and miRNA biogenesis pathway genes were evaluated by qRT-PCR in in vivo (n = 8) and IVF (n = 4) embryos. Results: The expression levels of let-7a and Stat3 were significantly reduced in IVF blastocyst when compared with in vivo (p =.004 and p =.009, respectively). Nevertheless, the IVF procedure did not influence the expression levels of miRNA biogenesis pathway components in 8-cell and blastocyst embryos. Conclusions: Downregulation of let-7a and developmental related transcription factor, Stat3, in IVF mouse blastocysts may affect preimplantation development and implantation of embryos. Moreover, the genes of the miRNA biogenesis pathway were not changed in preimplantation mouse embryos through the IVF procedure. © 2019 Wiley Periodicals, Inc.