Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Superovulation Affects the Gene Expression Patterns of Mice Oocytes and Preimplantation Embryos Produced by Different Assisted Reproductive Technologies Publisher



Jahanbakhshasl E1 ; Salehi M2, 3 ; Ghaffarinovin M1 ; Kato Y4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Biology and Anatomical Science, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  3. 3. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Laboratory of Animal Reproductive, College of Agriculture, KINDAI University, Nara, Japan

Source: International Journal of Women's Health and Reproduction Sciences Published:2018


Abstract

Objectives: In this experiment, possible effect of superovulation on important molecular profiles of oocytes and embryos were examined in mice regarding their development. Materials and Methods: A total of 120 metaphase II oocytes were produced by super or spontaneous ovulation. Blastocyst-stage embryos were produced after in vitro fertilization (IVF) and in vivo fertilization. Blastocysts that have been obtained from naturally mated female without gonadotrophin treatment were also used as controls. Using real-time polymerase chain reaction (PCR), the mRNA expression patterns of Bone morphogenesis protein (Bmp 15(, hepatoma-derived growth factor (Hdgf), DNA methyl transpherase-1 (Dnmt-1), developmental pluripotency associated 3 (Dppa3), and zinc finger protein 57 (Zfp57)genes were compared between superovulated and spontaneously ovulated oocytes; the expression patterns of H19 and small nuclear ribonucleoprotein N)Snrpn(imprinting genes between embryos produced from these oocytes were also compared. Results: The results of the study showed that expression of five maternal effect genes (Bmp15, Hdgf, Dnmt-1, Dppa3, and Zfp57) in superovulated oocytes were significantly reduced (P<0.05). However, the rates of cleavage to the blastocyst stages were not significantly different (P<0.05) between superovulated and naturally ovulated oocytes in cultured embryos. Moreover, superovulation disturbed the expression patterns of imprinting genes (H19 and Snrpn) in single blastocysts obtained by different assisted reproductive techniques (ARTs). Conclusions: The findings indicated that superovulation affected the molecular characteristics of ART conceived embryos. © 2018 The Author (s).
Related Docs
1. Methylation Status of H19/Igf2 Differentially Methylated Region in in Vitro Human Blastocysts Donated by Healthy Couples, Iranian Biomedical Journal (2017)
2. Vitrification at Day3 Stage Appears Not to Affect the Methylation Status of H19/Igf2 Differentially Methylated Region of In Vitro Produced Human Blastocysts, Cryobiology (2016)
3. Interfering Effects of in Vitro Fertilization and Vitrification on Expression of Gtl2 and Dlk1 in Mouse Blastocysts, International Journal of Fertility and Sterility (2020)
4. Expression of Mir-302 in Human Embryo Derived From In-Vitro Matured Oocyte, International Journal of Reproductive BioMedicine (2019)
5. Vitrification and in Vitro Culture Had No Adverse Effect on the Follicular Development and Gene Expression of Stimulated Human Ovarian Tissue, Journal of Obstetrics and Gynaecology Research (2018)
6. Does Sperm Dna Fragmentation Affect the Developmental Potential and the Incidence of Apoptosis Following Blastomere Biopsy?, Systems Biology in Reproductive Medicine (2016)
Experts (# of related papers)
Ashraf Moini (2)
Mina Naghi Jafarabadi (1)