Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Roles of Sertoli Cells in Fate Determinations of Spermatogonial Stem Cells



Khanehzad M1 ; Abolhasani F1 ; Koruji SM1 ; Kashani IR1 ; Aliakbari F1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Anatomy, Medical School, Tehran University of Medical Sciences, Tehran, Iran

Source: Tehran University Medical Journal Published:2016

Abstract

Background: Spermatogenesis is a complex and highly organized process of proliferation and differentiation of spermatogonial stem cells. Spermatogonial stem cells (SSCs) as a unique stem cell have the potential to self-renewal, differentiation and transmit genetic information to the next generation and play a vital role in maintaining fertility. Sertoli cells as the only somatic cells within the seminiferous epithelium play central roles in the formation of niche and balance between self-renewal and differentiation by secrete many growth factors. Given the importance and widespread use of SSCs, particularly in the treatment of infertility, the aim of this study was to create an optimal environment for the proliferation of SSCs. So we decided to study of undifferentiated (ID4) and differentiated (c-Kit) gene expression in SSCs followed by co-culture with Sertoli cells for a one-month. Methods: This experimental study was conducted from November 2013 to December 2014 in Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, on immature NMRI mouse (6-3 days old). Initially, Sertoli cells and SSCs were isolated from neonates mouse testes during the two-step enzymatic digestion characteristics Sertoli cells with vimentin marker and SSCs with promyelocytic leukemia zinc-finger (PLZF) marker were confirmed. Then SSCs were cultured in two groups: co-culture with Sertoli and without co-culture (control). Undifferentiated (ID4) and differentiation (c-Kit) gene expression were evaluated by Real-time PCR technique. Results: Spermatogonial stem cells purity was obtained 66.91% by flow cytometry. The relative expression levels of gene ID4 in co-culture group at the end of each week, compared to the control group showed a significant increase (P<0.05). While the expression of this gene significantly decreased in each group over time (P<0.05). The results of the comparison of the relative expression of c-Kit gene in co-culture group are indicated significant decrease than the control group at the end of each week (P<0.05). In addition, this gene expression was showed significant increase in each group individually over time (P<0.05) ID4 gene expression showed a significant (P<0.05) increase toward the control group, while in the expression of c-Kit was observed a significant (P<0.05) decrease compared with the control group at the end of each week. Conclusion: According to the results of this study, co-culture with Sertoli cells maintains SSCs in the prolifration stage for long-term, so can be used to optimize the culture medium at the clinic. © 2016, Tehran University of Medical Sciences. All rights reserved.
Related Docs
View other Related Docs
1. In Vitro Effects of Melatonin on Colonization of Neonate Mouse Spermatogonial Stem Cells, Systems Biology in Reproductive Medicine (2017)
2. The Effects of Melatonin on Colonization of Neonate Spermatogonial Mouse Stem Cells in a Three-Dimensional Soft Agar Culture System, Stem Cell Research and Therapy (2017)
3. The Epigenetic Assessment of Human Spermatogenic Cells Derived From Obstructive Azoospermic Patients in Different Culture Systems, Urology Journal (2021)
4. Use of Alginate Hydrogel to Improve Long-Term 3D Culture of Spermatogonial Stem Cells: Stemness Gene Expression and Structural Features, Zygote (2021)
5. In Vitro Spermatogenesis in Artificial Testis: Current Knowledge and Clinical Implications for Male Infertility, Cell and Tissue Research (2023)
6. Sertoli Cell Condition Medium Can Induce Germ Like Cells From Bone Marrow Derived Mesenchymal Stem Cells, Iranian Journal of Basic Medical Sciences (2016)
7. Advances of Three-Dimensional (3D) Culture Systems for in Vitro Spermatogenesis, Stem Cell Research and Therapy (2023)
8. Inductive Role of Sustentacular Cells (Sertoli Cells) Conditioned Medium on Bone Marrow Derived Mesenchymal Stem Cells; [Papel Inductivo Del Medio Acondicionado De Celulas Sustentaculares (Celulas De Sertoli) En Las Celulas Madre Mesenquimales Derivadas De La Medula Osea], International Journal of Morphology (2017)
Experts (# of related papers)
View all Related Experts
Tayebeh Rastegar (10)
Mehdi Abbasi (10)
Other Related Docs
9. Differentiation of Spermatogonial Stem Cells by Soft Agar Three-Dimensional Culture System, Artificial Cells# Nanomedicine and Biotechnology (2019)
10. Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of Pcl/Gel Nanofibers; [Proliferacion Y Diferenciacion De Celulas Madre Espermatogonicas De Raton En Una Superficie Tridimensional Compuesta De Nanofibras Pcl/Gel], International Journal of Morphology (2019)
11. Isolation, Culture and Characterization of Human Sertoli Cells by Flow Cytometry: Development of Procedure, Journal of Reproduction and Infertility (2017)
12. Alteration of Spermatogenesis Following Spermatogonial Stem Cells Transplantation in Testicular Torsion-Detorsion Mice, Journal of Assisted Reproduction and Genetics (2016)
13. Organ Culture of Seminiferous Tubules Using a Modified Soft Agar Culture System, Stem Cell Research and Therapy (2018)
14. The Interaction Between Sertoli Cells and Luekemia Inhibitory Factor on the Propagation and Differentiation of Spermatogonial Stem Cells in Vitro, International Journal of Reproductive BioMedicine (2015)
15. The Expression of Tlr2 and Tlr3 in Sertoli Cells of Azoospermic Patients, Cell Journal (2017)
16. Antioxidant Effect of Melatonin on Proliferation, Apoptosis, and Oxidative Stress Variables in Frozen-Thawed Neonatal Mice Spermatogonial Stem Cells, Biopreservation and Biobanking (2022)
17. Roles for Osteocalcin in Proliferation and Differentiation of Spermatogonial Cells Cocultured With Somatic Cells, Journal of Cellular Biochemistry (2019)
18. Sertoli Cell–Conditioned Medium Induces Differentiation of Bone Marrow–Derived Mesenchymal Stem Cells to Male Germ-Like Cells in Busulfan-Induced Azoospermic Mouse Model, Reproductive Sciences (2024)
19. Genetic and Epigenetic Evaluation of Human Spermatogonial Stem Cells Isolated by Macs in Different Two and Three-Dimensional Culture Systems, Cell Journal (2022)
20. Fsh Regulates Ra Signaling to Commit Spermatogonia Into Differentiation Pathway and Meiosis, Reproductive Biology and Endocrinology (2021)
21. Efficiency of Colony Formation and Differentiation of Human Spermatogenic Cells in Two Different Culture Systems, Reproductive Biology (2018)
22. Three-Dimensional Co-Culture of Human Spermatogonial Stem Cells With Sertoli Cells in Soft Agar Culture System Supplemented by Growth Factors and Laminin, Acta Histochemica (2020)
23. Silk Nanofibrous Electrospun Scaffold Amplifies Proliferation and Stemness Profile of Mouse Spermatogonial Stem Cells, Regenerative Engineering and Translational Medicine (2022)
24. Effect of Mir-30A-5P on Apoptosis, Colonization, and Oxidative Stress Variables in Frozen-Thawed Neonatal Mice Spermatogonial Stem Cells, Biopreservation and Biobanking (2021)
25. Testicular Mirnas in Relation to Spermatogenesis, Spermatogonial Stem Cells and Cancer/Testis Genes, Scientific African (2019)
26. Xenotransplantation Assessment: Morphometric Study of Human Spermatogonial Stem Cells in Recipient Mouse Testes, Andrologia (2015)
27. Isolation, Identification and Differentiation of Human Spermatogonial Cells on Three-Dimensional Decellularized Sheep Testis, Acta Histochemica (2020)
28. Tissue Engineering Studies in Male Infertility Disorder, Human Fertility (2023)
29. Roles for Kisspeptin in Proliferation and Differentiation of Spermatogonial Cells Isolated From Mice Offspring When the Cells Are Cocultured With Somatic Cells, Journal of Cellular Biochemistry (2019)
30. Morphological and Ultrastructural Studies of Human Spermatogonial Stem Cells From Patients With Maturation Arrest, Andrologia (2017)
31. Transplantation of Spermatogonial Stem Cells Suspension Into Rete Testis of Azoospermia Mouse Model, Urology Journal (2018)
32. Transplantation of Spermatogonial Stem Cells Suspension Into Rete Testis of Azoospermia Mouse Model, Urology Journal (2018)
33. Cryoprotective Effect of Pentoxifylline on Spermatogonial Stem Cell During Transplantation Into Azoospermic Torsion Mouse Model, Reproductive Sciences (2022)
34. Differentiation of Human Primary Testicular Cells in the Presence of Scf Using the Organoid Culture System, Artificial Organs (2023)
35. Effect of a Freezing Medium Containing Melatonin on Markers of Pre-Meiotic and Post-Meiotic Spermatogonial Stem Cells (Sscs) After Transplantation in an Azoospermia Mouse Model Due to Testicular Torsion, Reproductive Sciences (2021)
36. Gene and Histomorphology Alteration Analysis in Spermatogenesis Arrest Mouse Model: A Probable Novel Approach for Infertility, Central European Journal of Urology (2021)
37. Improving the Process of Spermatogenesis in Azoospermic Mice Using Spermatogonial Stem Cells Co-Cultured With Epididymosomes in Three-Dimensional Culture System, Life Sciences (2022)
38. Advances in Cryopreservation of Spermatogonial Stem Cells and Restoration of Male Fertility, Microscopy Research and Technique (2016)
39. Melatonin in Cryopreservation Media Improves Transplantation Efficiency of Frozen–Thawed Spermatogonial Stem Cells Into Testes of Azoospermic Mice, Stem Cell Research and Therapy (2022)