Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of Pcl/Gel Nanofibers; [Proliferacion Y Diferenciacion De Celulas Madre Espermatogonicas De Raton En Una Superficie Tridimensional Compuesta De Nanofibras Pcl/Gel]

Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of Pcl/Gel Nanofibers; [Proliferacion Y Diferenciacion De Celulas Madre Espermatogonicas De Raton En Una Superficie Tridimensional Compuesta De Nanofibras Pcl/Gel] Publisher

Talebi A¹ ; Sadighi Gilani MA² ; Koruji M³ ; Ai J⁴ ; Navid S¹ ; Rezaie MJ⁵ ; Jabari A¹ ; Movassagh SA¹ ; Khadivi F¹ ; Salehi M⁶ ; Hoshino Y⁷ ; Abbasi M¹

Show Affiliations

1. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Urology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
3. Cellular and Molecular Research Center & Department of Anatomical Sciences, Iran University of Medical Sciences, Tehran, Iran
4. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
5. Department of Embryology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
6. Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
7. Graduate School of Biosphere Science, Hiroshima University, Hiroshima, Japan

Source: International Journal of Morphology Published:2019

Abstract

Spermatogonial stem cells (SSCs) have self-renewal and differentiation capacity essential for sperm production throughout the male reproductive life. The electrospun polycaprolactone/gelatin (PCL/Gel) nanofibrous scaffold mimics important features of the extracellular matrix (ECM), which can provide a promising technique for the proliferation and differentiation of SSCs in vitro. The goal of the present study was to investigate the effects of PCL/Gel nanofibrous scaffold on the propagation and differentiation of neonate mouse SSCs (mSSCs). mSSCs were enzymatically isolated, and the cells were purified by differential plating method and seeded on scaffold. After 2 weeks, viability, colony number and diameter, and expression of specific spermatogonial cell genes were investigated. After mSSCs propagation, the cells were cultivated in a differentiation medium on the scaffold for another 2 weeks, and differentiating cells were analyzed by real-time PCR. The number of mSSC colony (P<0.01) and expression levels of specific spermatogonial genes Plzf and Inga6 (P<0.01) and also differentiation genes c-Kit, Tp1 and Ptm1 (P<0.05) were higher in scaffold group compared with control during the culture period. We conclude that mSSCs can be expanded and can differentiate toward spermatid cells on PCL/Gel nanofibrous scaffold with improved developmental parameters. © 2019, Universidad de la Frontera. All rights reserved.

Related Docs

View other Related Docs

1. Tissue Engineering Studies in Male Infertility Disorder, Human Fertility (2023)

2. Advances of Three-Dimensional (3D) Culture Systems for in Vitro Spermatogenesis, Stem Cell Research and Therapy (2023)

3. In Vitro Spermatogenesis in Artificial Testis: Current Knowledge and Clinical Implications for Male Infertility, Cell and Tissue Research (2023)

4. Advanced Bioengineering of Male Germ Stem Cells to Preserve Fertility, Journal of Tissue Engineering (2021)

5. The Effects of Melatonin on Colonization of Neonate Spermatogonial Mouse Stem Cells in a Three-Dimensional Soft Agar Culture System, Stem Cell Research and Therapy (2017)

6. Isolation, Identification and Differentiation of Human Spermatogonial Cells on Three-Dimensional Decellularized Sheep Testis, Acta Histochemica (2020)

7. Improving the Process of Spermatogenesis in Azoospermic Mice Using Spermatogonial Stem Cells Co-Cultured With Epididymosomes in Three-Dimensional Culture System, Life Sciences (2022)

8. Three-Dimensional Co-Culture of Human Spermatogonial Stem Cells With Sertoli Cells in Soft Agar Culture System Supplemented by Growth Factors and Laminin, Acta Histochemica (2020)

Experts (# of related papers)

View all Related Experts

Mehdi Abbasi (11)

Tayebeh Rastegar (9)

Style	Citing Format
MLA	Talebi A, et al.. "Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of Pcl/Gel Nanofibers; [Proliferacion Y Diferenciacion De Celulas Madre Espermatogonicas De Raton En Una Superficie Tridimensional Compuesta De Nanofibras Pcl/Gel]." International Journal of Morphology, vol. 37, no. 3, 2019, pp. 1132-1141.
APA	Talebi A, Sadighi Gilani MA, Koruji M, Ai J, Navid S, Rezaie MJ, Jabari A, Movassagh SA, Khadivi F, Salehi M, Hoshino Y, Abbasi M (2019). Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of Pcl/Gel Nanofibers; [Proliferacion Y Diferenciacion De Celulas Madre Espermatogonicas De Raton En Una Superficie Tridimensional Compuesta De Nanofibras Pcl/Gel]. International Journal of Morphology, 37(3), 1132-1141.
Chicago	Talebi A, Sadighi Gilani MA, Koruji M, Ai J, Navid S, Rezaie MJ, Jabari A, et al.. "Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of Pcl/Gel Nanofibers; [Proliferacion Y Diferenciacion De Celulas Madre Espermatogonicas De Raton En Una Superficie Tridimensional Compuesta De Nanofibras Pcl/Gel]." International Journal of Morphology 37, no. 3 (2019): 1132-1141.
Harvard	Talebi A et al. (2019) 'Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of Pcl/Gel Nanofibers; [Proliferacion Y Diferenciacion De Celulas Madre Espermatogonicas De Raton En Una Superficie Tridimensional Compuesta De Nanofibras Pcl/Gel]', International Journal of Morphology, 37(3), pp. 1132-1141.
Vancouver	Talebi A, Sadighi Gilani MA, Koruji M, Ai J, Navid S, Rezaie MJ, et al.. Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of Pcl/Gel Nanofibers; [Proliferacion Y Diferenciacion De Celulas Madre Espermatogonicas De Raton En Una Superficie Tridimensional Compuesta De Nanofibras Pcl/Gel]. International Journal of Morphology. 2019;37(3):1132-1141.
BibTex	@article{ author = {Talebi A and Sadighi Gilani MA and Koruji M and Ai J and Navid S and Rezaie MJ and Jabari A and Movassagh SA and Khadivi F and Salehi M and Hoshino Y and Abbasi M}, title = {Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of Pcl/Gel Nanofibers; [Proliferacion Y Diferenciacion De Celulas Madre Espermatogonicas De Raton En Una Superficie Tridimensional Compuesta De Nanofibras Pcl/Gel]}, journal = {International Journal of Morphology}, volume = {37}, number = {3}, pages = {1132-1141}, year = {2019} }
RIS	TY - JOUR AU - Talebi A AU - Sadighi Gilani MA AU - Koruji M AU - Ai J AU - Navid S AU - Rezaie MJ AU - Jabari A AU - Movassagh SA AU - Khadivi F AU - Salehi M AU - Hoshino Y AU - Abbasi M TI - Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of Pcl/Gel Nanofibers; [Proliferacion Y Diferenciacion De Celulas Madre Espermatogonicas De Raton En Una Superficie Tridimensional Compuesta De Nanofibras Pcl/Gel] JO - International Journal of Morphology VL - 37 IS - 3 SP - 1132 EP - 1141 PY - 2019 ER -

Style

Citing Format

MLA

Talebi A, et al.. "Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of Pcl/Gel Nanofibers; [Proliferacion Y Diferenciacion De Celulas Madre Espermatogonicas De Raton En Una Superficie Tridimensional Compuesta De Nanofibras Pcl/Gel]." International Journal of Morphology, vol. 37, no. 3, 2019, pp. 1132-1141.

APA

Talebi A, Sadighi Gilani MA, Koruji M, Ai J, Navid S, Rezaie MJ, Jabari A, Movassagh SA, Khadivi F, Salehi M, Hoshino Y, Abbasi M (2019). Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of Pcl/Gel Nanofibers; [Proliferacion Y Diferenciacion De Celulas Madre Espermatogonicas De Raton En Una Superficie Tridimensional Compuesta De Nanofibras Pcl/Gel]. International Journal of Morphology, 37(3), 1132-1141.

Chicago

Talebi A, Sadighi Gilani MA, Koruji M, Ai J, Navid S, Rezaie MJ, Jabari A, et al.. "Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of Pcl/Gel Nanofibers; [Proliferacion Y Diferenciacion De Celulas Madre Espermatogonicas De Raton En Una Superficie Tridimensional Compuesta De Nanofibras Pcl/Gel]." International Journal of Morphology 37, no. 3 (2019): 1132-1141.

Harvard

Talebi A et al. (2019) 'Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of Pcl/Gel Nanofibers; [Proliferacion Y Diferenciacion De Celulas Madre Espermatogonicas De Raton En Una Superficie Tridimensional Compuesta De Nanofibras Pcl/Gel]', International Journal of Morphology, 37(3), pp. 1132-1141.

Vancouver

Talebi A, Sadighi Gilani MA, Koruji M, Ai J, Navid S, Rezaie MJ, et al.. Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of Pcl/Gel Nanofibers; [Proliferacion Y Diferenciacion De Celulas Madre Espermatogonicas De Raton En Una Superficie Tridimensional Compuesta De Nanofibras Pcl/Gel]. International Journal of Morphology. 2019;37(3):1132-1141.

BibTex

@article{ author = {Talebi A and Sadighi Gilani MA and Koruji M and Ai J and Navid S and Rezaie MJ and Jabari A and Movassagh SA and Khadivi F and Salehi M and Hoshino Y and Abbasi M}, title = {Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of Pcl/Gel Nanofibers; [Proliferacion Y Diferenciacion De Celulas Madre Espermatogonicas De Raton En Una Superficie Tridimensional Compuesta De Nanofibras Pcl/Gel]}, journal = {International Journal of Morphology}, volume = {37}, number = {3}, pages = {1132-1141}, year = {2019} }

RIS

TY - JOUR
AU - Talebi A
AU - Sadighi Gilani MA
AU - Koruji M
AU - Ai J
AU - Navid S
AU - Rezaie MJ
AU - Jabari A
AU - Movassagh SA
AU - Khadivi F
AU - Salehi M
AU - Hoshino Y
AU - Abbasi M
TI - Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of Pcl/Gel Nanofibers; [Proliferacion Y Diferenciacion De Celulas Madre Espermatogonicas De Raton En Una Superficie Tridimensional Compuesta De Nanofibras Pcl/Gel]
JO - International Journal of Morphology
VL - 37
IS - 3
SP - 1132
EP - 1141
PY - 2019
ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract