Loop Mediated Isothermal Amplification Detection of Candida Albicans in Bal Samples of Suspected Tuberculosis Patients



Goodarzi M¹ ; Shahhosseiny MH^{2, 3} ; Bayat M¹ ; Hashemi SJ⁴ ; Ghahri M⁵ Show Affiliations
Source: Biochemical and Cellular Archives Published:2015

Abstract

With the increase in patients suffering from immune deficiency infections also increased pulmonary fungi and even in people in defect immune system caused fatal and lethal candidiasis. The timely diagnosis of pulmonary candidiasis is one of the problems has been detected. The purpose of this study correct and rapid diagnosis molecular Candida albicans in the samples of bronchoalveolar lavage (BAL) that can help measure suitable treatment. LAMP test Coptimized on the basis of alfa INT1 gene and then limited of detection (LOD) and specificity evaluated. Samples were the bronchoalveolar lavage suspected of tuberculosis reviews for TB disease negative have been reported. DNA extraction carried out by standard phenol/chloroform method on samples and LAMP test was done. In the specificity test with selected primers no product was not observed with different DNA samples, that indicating the high specificity of the primers and the LOD of LAMP tests was found to be approximately 100 CFU. The review of the 50 samples that who were negative for tuberculosis, 6 cases (12%) by the LAMP test was positive for Candida albicans. LAMP test is a rapid, sensitive and specific method and also low cost for diagnosis of systemic candidiasis in samples such as BAL in laboratory centers. © 2015, Connect Journal.