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Biocontrol Effect of Kluyveromyces Lactis on Aflatoxin Expression and Production in Aspergillus Parasiticus Publisher Pubmed



Ghanbari R1 ; Rezaie S2 ; Noorbakhsh F3 ; Khaniki GJ1 ; Soleimani M1 ; Aghaee EM1
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Authors Affiliations
  1. 1. Food Safety and Hygiene Division, Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Poursina St., Qods Ave., Tehran, Iran
  2. 2. Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Poursina St., Qods Ave., Tehran, Iran
  3. 3. Department of Microbiology, Biological Science College, Islamic Azad University, Varamin-Pishva Branch, 9 Dey Square, Pishva road, Varamin, Iran

Source: FEMS Microbiology Letters Published:2019


Abstract

Aspergillus parasiticus is one of the most common fungi able to produce aflatoxins, which are naturally occurring carcinogenic substances. This study evaluated the effects of the safe yeast, Kluyveromyces lactis, on fungal growth, aflatoxin production and expression of aflR gene in A. parasiticus. Antifungal susceptibility was evaluated by exposing A. parasiticus to different amounts of K. lactis, and aflatoxin production was measured using high-performance liquid chromatography. Expression of the aflR gene was determined by measuring the cognate aflR mRNA level by quantitative real-time reverse-transcription polymerase chain reaction assay. The growth of A. parasiticus was inhibited by 7 days of incubation at 30.C with a minimum population of 1.5 105 CFU/ml of K. lactis, which also suppressed expression of the A. parasiticus aflR gene, reducing the total production of aflatoxins by 97.9% and aflatoxins B1, B2, G1 and G2 by 97.8, 98.6, 98 and 94%, respectively. Accordingly, K. lactis could be considered as a potential biocontrol agent against toxigenic molds in food and animal feed. © 2019 FEMS.
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1. The Inhibitory Effect of Lactic Acid Bacteria on Aflatoxin Production and Expression of Aflr Gene in Aspergillus Parasiticus, Journal of Food Safety (2018)
2. Investigation the Effects of Lactobacillus Acidophilus and Lactobacillus Casei on Aflr Gene Expression in Aspergillus Parasiticus by Real Time-Pcr, Iranian Journal of Public Health (2016)
3. Antifungal Activity and Aflatoxin Degradation of Bifidobacterium Bifidum and Lactobacillus Fermentum Against Toxigenic Aspergillus Parasiticus, Open Microbiology Journal (2016)
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