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Resistance to Chlorhexidine and Benzalkonium Chloride in E. Coli St131, A. Baumannii, and P. Aeruginosa Isolates Publisher Pubmed



Farahani HE1 ; Asgharzadeh S2 ; Golmoradizadeh R1 ; Akrami S2 ; Ghaznavirad E3 ; Mirkalantari S1 ; Masjedianjazi F1
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Medical Laboratory Sciences, Para Medicine Faculty, Arak University of Medical Sciences, Arak, Iran

Source: Clinical Laboratory Published:2024


Abstract

Background: The goal was to assess the antimicrobial efficacy of two commonly used biocides, chlorhexidine, and benzalkonium chloride, against MDR isolates of Pseudomonas aeruginosa, Acinetobacter baumannii, and Escherichia coli ST131, as well as the prevalence of resistance genes. Methods: MIC of chlorhexidine and benzalkonium chloride and their effects on both the planktonic phase and biofilm were determined. Finally, the presence of genes responsible for resistance to quaternary ammonium compounds was investigated by PCR. Results: No significant relationship was observed between the presence of resistance genes and different concentrations of quaternary ammonium compounds (benzalkonium chloride). There was no association between biofilm formation and the presence of resistance genes. Conclusions: Chlorhexidine digluconate and benzalkonium chloride at appropriate concentrations could prevent biofilm formation. © 2024 Verlag Klinisches Labor GmbH. All rights reserved.