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Combination of Anti-Mir19a-3P Polyplex Plus Doxorubicin for Breast Cancer in 2D Culture and Apoptosis Assay in 3D Spheroids in a Microwell Device Publisher



Kheiri Yeghaneh Azar B1, 2, 3 ; Nourbakhsh M4, 5 ; Nasiraee MR6 ; Mousavizadeh K7 ; Majd Z1, 3 ; Ajoudanian M8 ; Saeedi S9, 10 ; Vahabi A3, 9 ; Hamblin MR11 ; Karimi M2, 3, 9, 12, 13
Authors
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Authors Affiliations
  1. 1. Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Oncopathology Research Center, Iran University of Medical Sciences, Tehran, Iran
  4. 4. Finetech in Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Medical Biotechnology, Faculty of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  8. 8. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  9. 9. Department of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
  10. 10. Neuroscience Research Center, Iran University of Medical Sciences, Tehran, Iran
  11. 11. Laser Research Centre, Faculty of Health Science, University of Johannesburg, Doornfontein, 2028, South Africa
  12. 12. Research Center for Science and Technology in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  13. 13. Applied Biotechnology Research Centre, Tehran Medical Science, Islamic Azad University, Tehran, Iran

Source: Journal of Micromechanics and Microengineering Published:2024


Abstract

One of the most common cancers and a main cause of death worldwide among women is breast cancer (BC). Combination therapy is being widely investigated to reduce the dose of chemotherapy drugs, prevent the development of drug resistance, and improve treatment outcomes. Here we tested PEI-PBA-SAP-F15 (PPSF) polymeric nanoparticles to efficiently deliver a microRNA antagonist (anti-miR19a-3p) to BC cell lines. We evaluated the combination of anti-miR19a-3p plus doxorubicin (DOX) in both 2D and 3D cell cultures. We cultured 3D tumor spheroids in an innovative microfluidic device that was fabricated using a 3D printing system. The PPSF polyplexes had the correct size and zeta potential to efficiently transfer anti-miR19a-3p into MCF7 cells. The expression level of phosphatase and tensin homolog (PTEN), the attainment gene of microRNA-19a-3p was increased. PTEN up-regulation inhibited cell migration and caused cell cycle arrest. Apoptosis was also significantly induced with the combination treatment. Confocal microscopy studies revealed that the population of dead cells was in an important degree higher in MCF7 spheroids transfected with anti-miR19a-3p-PPSF plus DOX. © 2023 IOP Publishing Ltd.
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