Anti-Inflammatory Function of Apolipoprotein B-Depleted Plasma Is Impaired in Nonalcoholic Fatty Liver Disease Publisher Pubmed



Sarmadi N¹ ; Poustchi H² ; Yari FA¹ ; Radmard AR³ ; Karami S¹ ; Pakdel A¹ ; Shabani P^{1, 4} ; Khaleghian A¹ Show Affiliations
Source: PLoS ONE Published:2022

Abstract

Background Non-alcoholic fatty liver disease (NAFLD) is associated with an increased risk of cardiovascular events. HDL exerts various protective functions on the cardiovascular system including anti-inflammatory activity by suppressing adhesion molecules expression in inflammation-induced endothelial cells. This study was designed to search if the anti-inflammatory capacity of apolipoprotein B-depleted plasma (apoB-depleted plasma) is altered in NAFLD patients. Methods A total of 83 subjects including 42 NAFLD and 41 control subjects were included in this cross-sectional study. Anti-inflammatory function of HDL was determined as the ability of apoB-depleted plasma to inhibit tumor necrosis factor-α (TNF-α)-induced expression of adhesion molecules in human umbilical vein endothelial cells (HUVECs). Results Incubation of inflammation-stimulated HUVECs with the NAFLD patients' apo-B depleted plasma led to higher levels of expression of adhesion molecules compared to the control subjects' plasma samples, reflecting an impaired anti-inflammatory capacity of apoBdepleted plasma in the NAFLD patients. Impaired anti-inflammatory capacity of apoBdepleted plasma was correlated with fatty liver and obesity indices. After adjustment with obesity indices, the association of anti-inflammatory capacity of apoB-depleted plasma with NAFLD remained significant. Conclusion Impaired anti-inflammatory activity of apoB-depleted plasma was independently associated with NAFLD. © 2022 Sarmadi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.