A Sandwich-Type Electrochemical Immunosensor Based on in Situ Silver Deposition for Determination of Serum Level of Her2 in Breast Cancer Patients

A Sandwich-Type Electrochemical Immunosensor Based on in Situ Silver Deposition for Determination of Serum Level of Her2 in Breast Cancer Patients Publisher Pubmed

Shamsipur M¹ ; Emami M² ; Farzin L³ ; Saber R⁴

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1. Department of Chemistry, Razi University, Kermanshah, Iran
2. School of Chemistry, University College of Science, University of Tehran, Tehran, Iran
3. Radiation Application Research School, Nuclear Science and Technology Research Institute, Tehran, Iran
4. Department of Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Science, Tehran, Iran

Source: Biosensors and Bioelectronics Published:2018

Abstract

The sensitive quantification of Human Epidermal growth factor Receptor 2 (HER2), as a key prognostic tumor marker, plays a critical role in screening, early diagnosis and management of breast cancer. This paper describes a sandwich-type immunoassay with silver signal enhancement strategy for highly sensitive detection of HER2. For this purpose, the target capturing step was designed by functionalization of 3-aminopropyltrimethoxysilane coated magnetite nanoparticles with antibody (antiHER2/APTMS-Fe3O4), as a platform bioconjugate (PB), and immobilized at a bare GCE. Then, in the presence of label-free immunosensor, the PB was covered by magnetic gold nanoparticles self-assembled with thiolated antibodies (antiHER2/Hyd@AuNPs-APTMS-Fe3O4) containing chemically reduced silver ions, as a label bioconjugate (LB). Under optimum conditions, a linear relationship between the differential pulse voltammetric (DPV) stripping signal of silver and the logarithm of HER2 concentrations was obtained in the range of 5.0 × 10−4-50.0 ng mL−1 (R2 = 0.9906) with a detection limit of 2.0 × 10−5 ng mL−1. The effectiveness of this protocol was evaluated experimentally through employing of designed immunosensor for detection of the serum level of tumor marker. The good consistency of the results with those obtained by the enzyme-linked immunosorbent assay (ELISA) conventional method (p-value of < 0.05) showed that this immunosensor can be applied for the testing of HER2 in clinical samples of breast cancer patients. © 2017 Elsevier B.V.

Style	Citing Format
MLA	Shamsipur M, et al.. "A Sandwich-Type Electrochemical Immunosensor Based on in Situ Silver Deposition for Determination of Serum Level of Her2 in Breast Cancer Patients." Biosensors and Bioelectronics, vol. 103, no. , 2018, pp. 54-61.
APA	Shamsipur M, Emami M, Farzin L, Saber R (2018). A Sandwich-Type Electrochemical Immunosensor Based on in Situ Silver Deposition for Determination of Serum Level of Her2 in Breast Cancer Patients. Biosensors and Bioelectronics, 103(), 54-61.
Chicago	Shamsipur M, Emami M, Farzin L, Saber R. "A Sandwich-Type Electrochemical Immunosensor Based on in Situ Silver Deposition for Determination of Serum Level of Her2 in Breast Cancer Patients." Biosensors and Bioelectronics 103, no. (2018): 54-61.
Harvard	Shamsipur M et al. (2018) 'A Sandwich-Type Electrochemical Immunosensor Based on in Situ Silver Deposition for Determination of Serum Level of Her2 in Breast Cancer Patients', Biosensors and Bioelectronics, 103(), pp. 54-61.
Vancouver	Shamsipur M, Emami M, Farzin L, Saber R. A Sandwich-Type Electrochemical Immunosensor Based on in Situ Silver Deposition for Determination of Serum Level of Her2 in Breast Cancer Patients. Biosensors and Bioelectronics. 2018;103():54-61.
BibTex	@article{ author = {Shamsipur M and Emami M and Farzin L and Saber R}, title = {A Sandwich-Type Electrochemical Immunosensor Based on in Situ Silver Deposition for Determination of Serum Level of Her2 in Breast Cancer Patients}, journal = {Biosensors and Bioelectronics}, volume = {103}, number = {}, pages = {54-61}, year = {2018} }
RIS	TY - JOUR AU - Shamsipur M AU - Emami M AU - Farzin L AU - Saber R TI - A Sandwich-Type Electrochemical Immunosensor Based on in Situ Silver Deposition for Determination of Serum Level of Her2 in Breast Cancer Patients JO - Biosensors and Bioelectronics VL - 103 IS - SP - 54 EP - 61 PY - 2018 ER -

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