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Gold and Silver Bio/Nano-Hybrids-Based Electrochemical Immunosensor for Ultrasensitive Detection of Carcinoembryonic Antigen Publisher Pubmed



Akbari Nakhjavani S1, 2 ; Afsharan H2 ; Khalilzadeh B3, 4 ; Ghahremani MH1, 5 ; Carrara S6 ; Omidi Y2, 7
Authors
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Authors Affiliations
  1. 1. Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran
  3. 3. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  4. 4. Biosensors and Bioelectronics Research Center, Ardabil University of Medical Sciences, Ardabil, Iran
  5. 5. Department of Pharmacology-Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Integrated Circuit Laboratory, Ecole Polytechnique Federale de Lausanne, Switzerland
  7. 7. Department of Pharmaceutics, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

Source: Biosensors and Bioelectronics Published:2019


Abstract

Carcinoembryonic antigen (CEA) is an important oncomarker for the detection of breast cancer. For ultra-sensitive sensing of CEA with great specificity and accuracy, an innovative and reliable electrochemical immunosensor was developed using various nano-hybrids. A glassy carbon electrode (GC) was modified with thiolated graphene oxide (T-GO) to elevate the active surface area of the electrode. The streptavidin-coated gold nanoparticles (AuNPs) were used to increase the conductivity of the sensing area as well as the loading capacity of the biotinylated monoclonal antibody (mAb). A sandwich-on approach was developed to reach a low limit of detection (LOD). The biotinylated mAb, streptavidin coated silver nanoparticles (AgNPs) and horseradish peroxidase (HRP), altogether, formed the signaling probe of the proposed immunosensor. The electrochemical signal was significantly enhanced in the presence of hydroquinone (HQ) and hydrogen peroxide (H2O2). Under the optimized conditions, the proposed immunosensor presented an excellent performance in a linear range of 100 fg/mL to 5 pg/mL with a low detection limit of 75 fg/mL. The engineered immunosensor displayed excellent specificity for the detection of CEA even in the real human serum, upon which it is proposed for the early detection and monitoring of CEA in the clinic. © 2019 Elsevier B.V.