Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Expression of Mir-221, Mir-29A, Mir-155 and Mir-146A in Peripheral Blood Mononuclear Cell (Pbmc) in Hiv-1 Infected Patients Publisher Pubmed



Narouei A1 ; Rouzbahani NH2, 4 ; Ghanbari M3 ; Taj L4 ; Mohraz M4 ; Gholami MM4, 5 ; Baesi K6
Authors
Show Affiliations
Authors Affiliations
  1. 1. Genetic Department, Damghan Islamic Azad University, Damghan, Iran
  2. 2. Department of Medical Immunology, Faculty of Medicine, Aja University of Medical Sciences, Tehran, Iran
  3. 3. Department of Microbial Biotechnology, Faculty of Biological Science, Tehran North Branch, Islamic Azad University, Tehran, Iran
  4. 4. Iranian Research Center for HIV AIDS, Iranian Institute for Reduction of High-Risk Behaviors, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Medical Microbiology, Faculty of Medicine, Aja University of Medical Sciences, Tehran, Iran
  6. 6. Hepatitis and AIDS Department, Pasteur Institute of Iran, Tehran, Iran

Source: Infectious Disorders - Drug Targets Published:2021


Abstract

Aims and Objectives: In patients infected by HIV-1, some cellular biomarkers such as microRNAs have an important function in the suppression or progression of the disease. The aim of the current study is to evaluate the expression of mir-221, mir-29a, mir-155, and mir-146a in HIV-1 infected patients. Methods: The miRNAs of 60 HIV-1 infected patients (sample group) and 20 healthy controls (nor-mal group) were extracted from their peripheral mononuclear cells. We used TaqMan-based Real--time PCR for evaluation of expression mir-155, mir-221, mir-29a and mir-146a by the comparative method. To evaluate differences among the data, one-way ANOVA was used. The expression of mir-155 and mir-146a in HIV-1 patients (sample group) was down-regulated in comparison with healthy controls (normal group) with a confidence value (***p <0.001). In addition, in the sample group, the expression of mir-221 was downregulated compared to the normal group (***p <0.001). Results: There was no significant difference in expression mi-29a in the sample and control group. In the sample group, mir-221 had a low expression, and mir-29a had a high expression, respective-ly. According to the results of the current study and comparative studies, it seems that the microR-NA has an important role in the progression or suppression of HIV-1 infection. Conclusion: However, the data showed that besides other cellular and viral factor, these miRNAs could be used as a biomarker. However, the miRNAs field experts are in general agreement that more investigation is needed to use miRNAs as a biomarker in HIV. © 2021 Bentham Science Publishers.
Related Docs
1. Micrornas and Exosomes: Key Players in Hiv Pathogenesis, HIV Medicine (2020)
2. Micrornas 29, 150, 155, 223 Level and Their Relation to Viral and Immunological Markers in Hiv-1 Infected Naive Patients, Future Virology (2018)
3. Exosomes and Micrornas in Biomedical Science, Synthesis Lectures on Biomedical Engineering (2022)
4. A Negative Correlation Between Hsa-Mir29a-3P Level and Hiv-1 Viral Load in Human Serum; Potentiate Criteria for Patients Screening, Microbial Pathogenesis (2023)
Experts (# of related papers)
Hamid Reza Mirzaei (2)
Seyed Mohammad Jazayeri (1)