Differential Expression of Circulating Mir-21, Mir-142-3P and Mir-155 in Renal Transplant Recipients With Impaired Graft Function

Differential Expression of Circulating Mir-21, Mir-142-3P and Mir-155 in Renal Transplant Recipients With Impaired Graft Function Publisher Pubmed

Zununi Vahed S¹ ; Poursadegh Zonouzi A² ; Ghanbarian H³ ; Ghojazadeh M⁴ ; Samadi N⁵ ; Omidi Y⁶ ; Ardalan M¹

Show Affiliations

1. Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2. Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3. School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4. Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
5. School of Advanced Biomedical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
6. Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Science, Tabriz, Iran

Source: International Urology and Nephrology Published:2017

Abstract

Background: The discovery of circulating microRNAs (miRNAs), as potential noninvasive diagnostic biomarkers, has opened new avenues of research for identifying patients with chronic failure in renal transplantation. The present study aimed to investigate the expression levels of four immune-related miRNAs (miR-21, miR-31, miR-142-3p and miR-155) in plasma samples of renal recipients. Methods: The plasma expression levels of the miRNAs were evaluated by quantitative real-time PCR (qPCR) in 53 renal recipients with long-term stable allograft function, SGF (N = 27), and with biopsy-proven interstitial fibrosis and tubular atrophy (IFTA) (N = 26) and also healthy controls (N = 15). The possible correlation between clinical parameters and the circulating miRNAs and the receiver-operating characteristic (ROC) analysis were performed. Results: Our results showed that expression of miR-21 (p = 0.023), miR-142-3p (p = 0.048) and miR-155 (p = 0.005) was significantly upregulated in plasma samples of recipients with IFTA in comparison with SGF and healthy control groups. Concentration of miR-21 (∆Ct value) in plasma was negatively correlated with creatinine (r = −0.432, p = 0.028) and positively correlated with eGFR (r = 0.423, p = 0.031). The multivariate ROC curve analysis indicated that miR-21, miR-142-3p and miR-155 in plasma samples could discriminate almost most of the IFTA patients (area under curve = 0.802, sensitivity = 81%, specificity = 92%). Conclusion: Our data suggested that altered expression of miR-21, miR-142-3p and miR-155 in plasma samples may be associated with renal dysfunction and can be used for graft monitoring. © 2017, Springer Science+Business Media Dordrecht.

2. Altered Expression of Micrornas Following Chronic Allograft Dysfunction With Interstitial Fibrosis and Tubular Atrophy, Iranian Journal of Allergy# Asthma and Immunology (2015)

3. Differential Expression of Micrornas in Renal Transplant Patients With Acute T-Cell Mediated Rejection, Transplant Immunology (2015)

4. Melatonin Affects the Expression of Microrna-21: A Mini-Review of Current Evidence, Pathology Research and Practice (2024)

5. Epigenetics and Pathogenesis of Systemic Sclerosis; the Ins and Outs, Human Immunology (2018)

6. Microparticles in Kidney Diseases: Focus on Kidney Transplantation, Renal Replacement Therapy (2017)

7. Altered Microrna Expression and Immunosuppressive Cytokine Production by Regulatory T Cells of Ulcerative Colitis Patients, Immunological Investigations (2016)

Experts (# of related papers)

View all Related Experts

Ali Akbar Amirzargar (2)

Mir Saeed Yekaninejad (2)

Style	Citing Format
MLA	Zununi Vahed S, et al.. "Differential Expression of Circulating Mir-21, Mir-142-3P and Mir-155 in Renal Transplant Recipients With Impaired Graft Function." International Urology and Nephrology, vol. 49, no. 9, 2017, pp. 1681-1689.
APA	Zununi Vahed S, Poursadegh Zonouzi A, Ghanbarian H, Ghojazadeh M, Samadi N, Omidi Y, Ardalan M (2017). Differential Expression of Circulating Mir-21, Mir-142-3P and Mir-155 in Renal Transplant Recipients With Impaired Graft Function. International Urology and Nephrology, 49(9), 1681-1689.
Chicago	Zununi Vahed S, Poursadegh Zonouzi A, Ghanbarian H, Ghojazadeh M, Samadi N, Omidi Y, Ardalan M. "Differential Expression of Circulating Mir-21, Mir-142-3P and Mir-155 in Renal Transplant Recipients With Impaired Graft Function." International Urology and Nephrology 49, no. 9 (2017): 1681-1689.
Harvard	Zununi Vahed S et al. (2017) 'Differential Expression of Circulating Mir-21, Mir-142-3P and Mir-155 in Renal Transplant Recipients With Impaired Graft Function', International Urology and Nephrology, 49(9), pp. 1681-1689.
Vancouver	Zununi Vahed S, Poursadegh Zonouzi A, Ghanbarian H, Ghojazadeh M, Samadi N, Omidi Y, et al.. Differential Expression of Circulating Mir-21, Mir-142-3P and Mir-155 in Renal Transplant Recipients With Impaired Graft Function. International Urology and Nephrology. 2017;49(9):1681-1689.
BibTex	@article{ author = {Zununi Vahed S and Poursadegh Zonouzi A and Ghanbarian H and Ghojazadeh M and Samadi N and Omidi Y and Ardalan M}, title = {Differential Expression of Circulating Mir-21, Mir-142-3P and Mir-155 in Renal Transplant Recipients With Impaired Graft Function}, journal = {International Urology and Nephrology}, volume = {49}, number = {9}, pages = {1681-1689}, year = {2017} }
RIS	TY - JOUR AU - Zununi Vahed S AU - Poursadegh Zonouzi A AU - Ghanbarian H AU - Ghojazadeh M AU - Samadi N AU - Omidi Y AU - Ardalan M TI - Differential Expression of Circulating Mir-21, Mir-142-3P and Mir-155 in Renal Transplant Recipients With Impaired Graft Function JO - International Urology and Nephrology VL - 49 IS - 9 SP - 1681 EP - 1689 PY - 2017 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract