Evaluating the Mechanism Underlying Antitumor Effect of Interleukin 27 on B Cells of Chronic Lymphocytic Leukemia Patients Publisher Pubmed



Manouchehridoulabi E^{1, 2, 3} ; Abbaspour S^{1, 2} ; Rostami S⁴ ; Faranoush M⁵ ; Ghahramanfard F^{1, 2} ; Pak F¹ ; Barati M^{6, 7} ; Kokhaei P^{1, 2, 8} ; Momtaziborojeni AA^{9, 10}
Source: Journal of Cellular Physiology Published:2020

Abstract

Chronic lymphocyte leukemia (CLL) is a B-cell malignancy resisted to apoptosis. Recently, some studies indicated that cytokines such as interleukin 27 (IL-27) can reduce B-cell proliferation. The aim of this study is to evaluate the mechanism underlying the proapoptotic effect of IL-27 on B cells of patients with CLL in comparison with B cells of normal subjects. The effect of IL-27 on the antitumor activity of natural killer (NK) and T cells was also evaluated. Peripheral blood mononuclear cells (PBMCs) were isolated from 35 patients with CLL and 15 normal subjects. B cells and PBMCs were cocultured with IL-27 and B cells apoptosis to evaluate proliferation. Both messenger RNA and protein expression of IL-27 and IL-27 receptor were determined using flow cytometry and real-time polymerase chain reaction analysis. To evaluate the apoptotic effect of IL-27 on B cells of patients with CLL, Annexin V-FITC and 7-AAD (BioLegend) fluorescent dyes were used. In addition, the IL-27 effect on activation of T cell and NK cell was determined by determining CD96 molecule expression. IL-27 and IL-27 receptor expression in patients with CLL was significantly lower than that of normal subjects (p '.05). IL-27 enhanced apoptosis of B cells in patients with CLL (p '.05) but this effect was not significantly observed in B cells of normal subjects (p '.05). Consequently, IL-27 reduced the proliferation of B cells and enhanced NK cell activity (p '.05). IL-27, through inducing apoptosis, can exert an inhibitory effect on cancer B cells of CLL patients with minimal effect on normal B cells. © 2020 Wiley Periodicals LLC