The Crispr Growth Spurt: From Bench to Clinic on Versatile Small Rnas Publisher Pubmed



Bayat H^{1, 2} ; Omidi M^{1, 2} ; Rajabibazl M³ ; Sabri S⁴ ; Rahimpour A^{1, 2}
Source: Journal of Microbiology and Biotechnology Published:2017

Abstract

Clustered regulatory interspaced short palindromic repeats (CRISPR) in association with CRISPR-associated protein (Cas) is an adaptive immune system, playing a pivotal role in the defense of bacteria and archaea. Ease of handling and cost effectiveness make the CRISPR-Cas system an ideal programmable nuclease tool. Recent advances in understanding the CRISPR-Cas system have tremendously improved its efficiency. For instance, it is possible to recapitulate the chronicle CRISPR-Cas from its infancy and inaugurate a developed version by generating novel variants of Cas proteins, subduing off-target effects, and optimizing of innovative strategies. In summary, the CRISPR-Cas system could be employed in a number of applications, including providing model systems, rectification of detrimental mutations, and antiviral therapies. © 2017 by The Korean Society for Microbiology and Biotechnology.