Beta-Tubulin Gene in the Differentiation of Fusarium Species by Pcr-Rflp Analysis

Beta-Tubulin Gene in the Differentiation of Fusarium Species by Pcr-Rflp Analysis Pubmed

Nosratabadi M¹ ; Kachuei R² ; Rezaie S³ ; Harchegani AB⁴

Show Affiliations

1. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2. Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
3. Divisions of Molecular Biology, Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
4. Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

Source: Infezioni in Medicina Published:2018

Abstract

Fusarium species belong to one of the most important fungal groups in the medical, agricultural, and veterinary fields. This study aimed to evaluate the efficiency of PCR-RFLP analysis of the beta (β)-tubulin region for differentiating Fusarium species. A total of 107 strains of Fusarium spp. were studied, including isolates from environmental, clinical, and reference sources. The β-tubulin genes of all isolates were successfully amplified with primer pairs (T1 and T22). A PCR product of approximately 1400 base pairs was generated for each Fusarium sp. After evaluation of various enzymes, three restriction enzymes, namely Ban II, BsaWI, and HincII, were selected. Based on the selected enzymes, the isolated Fusarium spp. were categorized into 24 groups. In this study we were able to identify F. graminearum, F. culmorum, and F. cerealis through the proposed analyses as well as other pathogenically important species such as F. oxysporum and F. solani. Unlike all other similar previous studies, this study was able to differentiate among F. graminearum, F. culmorum, and F. cerealis. However, we were unable to differentiate F. ar-meniacum and F. acuminatum or F. sportrichioides from F. langsethiae. Hence, it is recommended that other genes must be evaluated to overcome the limitations of the β-tubulin gene in differentiating the above species. © 2018, EDIMES Edizioni Medico Scientifiche. All rights reserved.

2. In Vitro Antifungal Susceptibility Testing of Clinical and Environmental Fusarium Isolates in Iran, Archives of Clinical Infectious Diseases (2018)

3. A Case of Fungus Ball-Type Pansinusitis Due to Fusarium Proliferatum, Mycopathologia (2015)

4. Phylogenetic Relationship of Fusarium Species Isolated From Keratitis Using Tef1 and Rpb2 Gene Sequences, Iranian Journal of Microbiology (2022)

Experts (# of related papers)

View all Related Experts

Sassan Rezaie (1)

Roshanak Daei Ghazvini (1)

Style	Citing Format
MLA	Nosratabadi M, et al.. "Beta-Tubulin Gene in the Differentiation of Fusarium Species by Pcr-Rflp Analysis." Infezioni in Medicina, vol. 26, no. 1, 2018, pp. 52-60.
APA	Nosratabadi M, Kachuei R, Rezaie S, Harchegani AB (2018). Beta-Tubulin Gene in the Differentiation of Fusarium Species by Pcr-Rflp Analysis. Infezioni in Medicina, 26(1), 52-60.
Chicago	Nosratabadi M, Kachuei R, Rezaie S, Harchegani AB. "Beta-Tubulin Gene in the Differentiation of Fusarium Species by Pcr-Rflp Analysis." Infezioni in Medicina 26, no. 1 (2018): 52-60.
Harvard	Nosratabadi M et al. (2018) 'Beta-Tubulin Gene in the Differentiation of Fusarium Species by Pcr-Rflp Analysis', Infezioni in Medicina, 26(1), pp. 52-60.
Vancouver	Nosratabadi M, Kachuei R, Rezaie S, Harchegani AB. Beta-Tubulin Gene in the Differentiation of Fusarium Species by Pcr-Rflp Analysis. Infezioni in Medicina. 2018;26(1):52-60.
BibTex	@article{ author = {Nosratabadi M and Kachuei R and Rezaie S and Harchegani AB}, title = {Beta-Tubulin Gene in the Differentiation of Fusarium Species by Pcr-Rflp Analysis}, journal = {Infezioni in Medicina}, volume = {26}, number = {1}, pages = {52-60}, year = {2018} }
RIS	TY - JOUR AU - Nosratabadi M AU - Kachuei R AU - Rezaie S AU - Harchegani AB TI - Beta-Tubulin Gene in the Differentiation of Fusarium Species by Pcr-Rflp Analysis JO - Infezioni in Medicina VL - 26 IS - 1 SP - 52 EP - 60 PY - 2018 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract