Evaluation of Pcr-Reverse Line Blot Hybridization Assay for Simultaneous Identification of Medically Important Saprophytic Fungi

Evaluation of Pcr-Reverse Line Blot Hybridization Assay for Simultaneous Identification of Medically Important Saprophytic Fungi Publisher Pubmed

Agha Kuchak Afshari S¹ ; Rahimi H² ; Hashemi SJ¹ ; Daie Ghazvini R¹ ; Badali H³ ; Aghaei Gharehbolagh S¹ ; Rezaie S¹

Show Affiliations

1. Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2. Molecular Medicine Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
3. Department of Medical Mycology and Parasitology, Antimicrobial Resistance Research Center (ARRC), Mazandaran University of Medical Science, Sari, Iran

Source: Journal de Mycologie Medicale Published:2018

Abstract

Background: In immunocompromised patients suffering from invasive fungal infections, rapid identification of fungal species is important since the appropriate treatment is usually related to the responsible species. We describe here, an assay based on combination of PCR and reverse line blot hybridization (PCR/RLB) for differentiation causative agent of fungal infections. Materials and methods: We performed PCR/RLB assay on 10 reference strains, which include Aspergillus species (A. fumigatus, A. flavus, A. niger, A. terreus, and A. clavatus), Mucor circnelloides, Rhizopus oryzae, Alternaria alternata, Cladosporium herbarum, and Fusarium solani. Besides, twenty-two clinical specimens from patients with proven fungal infections were analyzed for the identification of species. The obtained results were then compared with the results of culture and sequence analysis. Results: The fungal species–specific oligonucleotide probes were able to distinguish between all species represented in this study with the exception of cross-reactivity between A. niger and A. fumigatus species. Two specimens, which were represented as mixed fungi in culture, were identified properly by this method. Results of the RLB assay were concordant with the culture and ITS sequencing results. Conclusion: Our result demonstrate that the RLB assay potentially is suitable for rapid and simultaneous identification of variety fungal pathogens directly from culture as well as from clinical specimens. © 2017 Elsevier Masson SAS

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Style	Citing Format
MLA	Agha Kuchak Afshari S, et al.. "Evaluation of Pcr-Reverse Line Blot Hybridization Assay for Simultaneous Identification of Medically Important Saprophytic Fungi." Journal de Mycologie Medicale, vol. 28, no. 1, 2018, pp. 173-179.
APA	Agha Kuchak Afshari S, Rahimi H, Hashemi SJ, Daie Ghazvini R, Badali H, Aghaei Gharehbolagh S, Rezaie S (2018). Evaluation of Pcr-Reverse Line Blot Hybridization Assay for Simultaneous Identification of Medically Important Saprophytic Fungi. Journal de Mycologie Medicale, 28(1), 173-179.
Chicago	Agha Kuchak Afshari S, Rahimi H, Hashemi SJ, Daie Ghazvini R, Badali H, Aghaei Gharehbolagh S, Rezaie S. "Evaluation of Pcr-Reverse Line Blot Hybridization Assay for Simultaneous Identification of Medically Important Saprophytic Fungi." Journal de Mycologie Medicale 28, no. 1 (2018): 173-179.
Harvard	Agha Kuchak Afshari S et al. (2018) 'Evaluation of Pcr-Reverse Line Blot Hybridization Assay for Simultaneous Identification of Medically Important Saprophytic Fungi', Journal de Mycologie Medicale, 28(1), pp. 173-179.
Vancouver	Agha Kuchak Afshari S, Rahimi H, Hashemi SJ, Daie Ghazvini R, Badali H, Aghaei Gharehbolagh S, et al.. Evaluation of Pcr-Reverse Line Blot Hybridization Assay for Simultaneous Identification of Medically Important Saprophytic Fungi. Journal de Mycologie Medicale. 2018;28(1):173-179.
BibTex	@article{ author = {Agha Kuchak Afshari S and Rahimi H and Hashemi SJ and Daie Ghazvini R and Badali H and Aghaei Gharehbolagh S and Rezaie S}, title = {Evaluation of Pcr-Reverse Line Blot Hybridization Assay for Simultaneous Identification of Medically Important Saprophytic Fungi}, journal = {Journal de Mycologie Medicale}, volume = {28}, number = {1}, pages = {173-179}, year = {2018} }
RIS	TY - JOUR AU - Agha Kuchak Afshari S AU - Rahimi H AU - Hashemi SJ AU - Daie Ghazvini R AU - Badali H AU - Aghaei Gharehbolagh S AU - Rezaie S TI - Evaluation of Pcr-Reverse Line Blot Hybridization Assay for Simultaneous Identification of Medically Important Saprophytic Fungi JO - Journal de Mycologie Medicale VL - 28 IS - 1 SP - 173 EP - 179 PY - 2018 ER -

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