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A Ratiometric Fluorescent Probe Based on Pim-1 Semiconducting Polymer Dots for Turn-Off-On Sensing and Bioimaging Publisher



Shamsipur M1 ; Feizi F1 ; Molaabasi F2 ; Shamsipur H3 ; Mousavi F1 ; Sedghi M4 ; Budd P5 ; Naderimanesh H4
Authors
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Authors Affiliations
  1. 1. Department of Chemistry, Razi University, Kermanshah, Iran
  2. 2. Biomaterials and Tissue Engineering Research Group, Department of Interdisciplinary Technologies, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran
  3. 3. Laser and Plasma Research Institute, Shahid Behehsti University, Tehran, Iran
  4. 4. Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modarres University, Tehran, Iran
  5. 5. Department of Chemistry, University of Manchester, Manchester, M13 9PL, United Kingdom

Source: Sensors and Actuators B: Chemical Published:2023


Abstract

Herein, we report a simple green platform based on a novel type of polymer dots (Pdots) as a sensitive and rapid “off-on” fluorescent probe for ratiometric Fe3+ and Cu2+ detection. The ratiometric probe has been designed using green-emitting PIM-1 Pdots and red-emitting PFTBT Pdots as detecting probes and internal stable standard probes, respectively. The dual-emission characteristics of PIM-1/PFTBT Pdots allowed us to apply ratiometric ion determination based on aggregation-caused quenching (ACQ) of PIM-1 Pdots at 490 nm, while the red emission intensity kept highly stable. The role of porosity and surface carboxylic groups of PIM-1 to achieve high sensitivity and selectivity using quantum-mechanical calculation and cellular imaging was successfully demonstrated so that the detection limit of 7.4 nM for Fe3+ and 14.8 nM for Cu2+ was obtained. The “on” process with excellent selectivity was realized by the introducing of glutathione (GSH) into PIM-1/PFTBT/Cu2+ and Cytochrome c (Cyt c) into PIM-1/PFTBT/Fe3+ with fluorescence recoveries of the blank sample 92 % and 91 %, respectively. The proposed strategy detects GSH and Cyt c with a good linearity ranging from 300 nM to 1800 nM and 10 nM to 120 nM by the limit of detection of 85.3 nM and 0.6 nM, respectively. This combinational off-on strategy provides a promising nanosensing platform for monitoring Fe3+ and Cu2+ in the environmental samples and introduces a sensitive probe with high biocompatibility for intracellular target imaging. © 2023 Elsevier B.V.
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