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Protective Potential of Conjugated P. Aeruginosa Lps –Plga Nanoparticles in Mice As a Nanovaccine Publisher Pubmed



Zanjani LS1 ; Shapoury R2 ; Dezfulian M1, 3 ; Mahdavi M4 ; Shafieeardestani M5
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran
  2. 2. Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran
  3. 3. Biotechnology Research Center, Karaj Branch, Islamic Azad University, Karaj, Iran
  4. 4. Pasteur Institute of Iran, Iran
  5. 5. Department of Rhiopharmacy, Faculty of Pharmacy, Tehran of Medical Science, Tehran, Iran

Source: Iranian Journal of Immunology Published:2020


Abstract

Background: Pseudomonas aeruginosa has an important role in nosocomial infections. Objective: To evaluate biological activity of the detoxified LPS (D-LPS) entrapped into Poly lactic-co-glycolic acid (PLGA) nanoparticles. Materials: LPS was extracted and detoxified from the P. aeruginosa strain PAO1. The D-LPS, conjugated to the PLGA nanoparticles with 1-ethyl-3-dimethyl aminopropyl carbodiimide (EDAC) and N-hydroxy-succinimide (NHS). The connection was evaluated by FTIR (Fourier transform infrared), Zetasizer, and Atomic Force Microscope (AFM). The BALB/c mice injected intramuscularly with the D-LPS-PLGA with two-week intervals and then challenged two weeks after the last immunization. The bioactivity of the induced specific antisera and cytokines responses against D-LPS-PLGA antigen was assessed by ELISA. Results: D-LPS-PLGA conjugation was confirmed by FTIR, Zetasizer, and AFM. The ELISA results showed that D-LPS was successful in the stimulation of the humoral immune response. The immune responses raised against the D-LPS-PLGA, significantly decreased bacterial titer in the spleen of the immunized mice after challenge with PAO1 strain in comparison with the control groups. Conclusion: The conjugation of the bacterial LPS to the PLGA nanoparticle increased their functional activity by decrease in bacterial dissemination and increase the killing of opsonized bacteria. © 2020, Shiraz University of Medical Sciences. All rights reserved.
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