Virus-Directed Synthesis of Emitting Copper Nanoclusters As an Approach to Simple Tracer Preparation for the Detection of Citrus Tristeza Virus Through the Fluorescence Anisotropy Immunoassay

Virus-Directed Synthesis of Emitting Copper Nanoclusters As an Approach to Simple Tracer Preparation for the Detection of Citrus Tristeza Virus Through the Fluorescence Anisotropy Immunoassay Publisher

Shokri E^{1, 2} ; Hosseini M^{2, 3} ; Sadeghan AA⁴ ; Bahmani A² ; Nasiri N⁵ ; Hosseinkhani S⁶

Show Affiliations

1. Department of Nanotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
2. Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran
3. Medical Biomaterials Research Center (MBRC), Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
4. Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
5. Department of Biotechnology and Plant Breeding, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran
6. Department of Biochemistry, Tarbiat Modares University, Tehran, Iran

Source: Sensors and Actuators# B: Chemical Published:2020

Abstract

Citrus Tristeza Virus (CTV) was detected by targeting the virus capsid protein, CP25, through a competitive fluorescence anisotropic immunoassay. In this assay, instead of using chromophore labeled tracers, a simple approach in which the target antigen serves both as the main target and tracer was developed. For this, CP25 was expressed in E.coli and the purified recombinant protein was manipulated for copper nanocluster synthesis. The resultant CP25/CuNCs optically showed quantum dot-like behavior and emitted light in a range of 535–715 nm. The CP25/CuNCs could also interact with the anti-CTV antibody and showed polarized emission. In this assay, the CP25 protein competes with the fluorescent CP25/CuNCs used as a tracer for immune-complex formation with the anti-CTV antibody. The results showed that the FA values decreased linearly from 400 pg/mL to 25 ng/mL with an increase in CP25 protein levels and the limit of detection (LOD) was 220 pg/mL. The procedure of this assay can be useful for the detection of low molecular weight proteins or peptides in general, due to the availability of various kinds of metal nanoclusters and synthesis protocols. © 2020 Elsevier B.V.

2. Sensitive Detection of S. Aureus Using Aptamer- and Vancomycin -Copper Nanoclusters As Dual Recognition Strategy, Food Chemistry (2021)

3. Oxidase-Like Catalytic Activity of Cys-Auncs Upon Visible Light Irradiation and Its Application for Visual Mirna Detection, Sensors and Actuators# B: Chemical (2018)

Style	Citing Format
MLA	Shokri E, et al.. "Virus-Directed Synthesis of Emitting Copper Nanoclusters As an Approach to Simple Tracer Preparation for the Detection of Citrus Tristeza Virus Through the Fluorescence Anisotropy Immunoassay." Sensors and Actuators# B: Chemical, vol. 321, no. , 2020, pp. -.
APA	Shokri E, Hosseini M, Sadeghan AA, Bahmani A, Nasiri N, Hosseinkhani S (2020). Virus-Directed Synthesis of Emitting Copper Nanoclusters As an Approach to Simple Tracer Preparation for the Detection of Citrus Tristeza Virus Through the Fluorescence Anisotropy Immunoassay. Sensors and Actuators# B: Chemical, 321(), -.
Chicago	Shokri E, Hosseini M, Sadeghan AA, Bahmani A, Nasiri N, Hosseinkhani S. "Virus-Directed Synthesis of Emitting Copper Nanoclusters As an Approach to Simple Tracer Preparation for the Detection of Citrus Tristeza Virus Through the Fluorescence Anisotropy Immunoassay." Sensors and Actuators# B: Chemical 321, no. (2020): -.
Harvard	Shokri E et al. (2020) 'Virus-Directed Synthesis of Emitting Copper Nanoclusters As an Approach to Simple Tracer Preparation for the Detection of Citrus Tristeza Virus Through the Fluorescence Anisotropy Immunoassay', Sensors and Actuators# B: Chemical, 321(), pp. -.
Vancouver	Shokri E, Hosseini M, Sadeghan AA, Bahmani A, Nasiri N, Hosseinkhani S. Virus-Directed Synthesis of Emitting Copper Nanoclusters As an Approach to Simple Tracer Preparation for the Detection of Citrus Tristeza Virus Through the Fluorescence Anisotropy Immunoassay. Sensors and Actuators# B: Chemical. 2020;321():-.
BibTex	@article{ author = {Shokri E and Hosseini M and Sadeghan AA and Bahmani A and Nasiri N and Hosseinkhani S}, title = {Virus-Directed Synthesis of Emitting Copper Nanoclusters As an Approach to Simple Tracer Preparation for the Detection of Citrus Tristeza Virus Through the Fluorescence Anisotropy Immunoassay}, journal = {Sensors and Actuators# B: Chemical}, volume = {321}, number = {}, pages = {-}, year = {2020} }
RIS	TY - JOUR AU - Shokri E AU - Hosseini M AU - Sadeghan AA AU - Bahmani A AU - Nasiri N AU - Hosseinkhani S TI - Virus-Directed Synthesis of Emitting Copper Nanoclusters As an Approach to Simple Tracer Preparation for the Detection of Citrus Tristeza Virus Through the Fluorescence Anisotropy Immunoassay JO - Sensors and Actuators# B: Chemical VL - 321 IS - SP - EP - PY - 2020 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract