Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Sensitive Detection of S. Aureus Using Aptamer- and Vancomycin -Copper Nanoclusters As Dual Recognition Strategy Publisher Pubmed



Bagheri Pebdeni A1 ; Mousavizadegan M1 ; Hosseini M1, 2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran
  2. 2. Medical Biomaterials Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Source: Food Chemistry Published:2021


Abstract

The proposed aptamer- and antibiotic-based dual detection sensor, combines copper nanoclusters (CuNCs) as an effective approach for the recognition and quantification of Staphylococcus aureus (S. aureus) as a pathogenic bacteria. A facile method for CuNCs based on vancomycin as the template using a fluorescence platform was proposed for the recognition of the S. aureus whole cells via antibiotic and aptamer. Using dual receptor functionalized CuNCs linked to vancomycin and a specific aptamer and during aggregation induce emission process enhanced fluorescence signal linearly with S. aureus concentrations between 102-108 CFU/mL, and the detection limit was 80 CFU/mL after 45 min as the optimum incubation time. Non-target bacteria generated negative results, proving the high specificity of the presented sensor. This strategy showed recoveries ranging 86%-98% in milk as real sample and can be used for the development of universal detection platforms for efficient and specific S. aureus detection with great potential applications for monitoring pathogenic bacteria. © 2021 Elsevier Ltd
Related Docs
View other Related Docs
1. Fluorometric Determination of Microrna Via Fret Between Silver Nanoclusters and Cdte Quantum Dots, Microchimica Acta (2017)
2. Biosensing Using Dna-Based Structures Integrated With Nanosheets, Microchemical Journal (2023)
3. Biomarker Sensing Using Luminescent Metal Nanoclusters, Luminescent Metal Nanoclusters: Synthesis# Characterization# and Applications (2022)
4. The Synthesis of Pt Doped Wo3 Nanosheets and Application on Colorimetric Detection of Cysteine by Naked Eye Using Response Surface Methodology for Optimization, Environmental Research (2022)
5. Visual Detection of Mirna Using Peroxidase-Like Catalytic Activity of Dna-Cuncs and Methylene Blue As Indicator, Clinica Chimica Acta (2018)
6. A Unique Fret Approach Toward Detection of Single-Base Mismatch Dna in Brca1 Gene, Materials Science and Engineering C (2019)
7. Challenges and Opportunities of Using Fluorescent Metal Nanocluster-Based Colorimetric Assays in Medicine, ACS Omega (2023)
8. Detection of Large Deletion in Human Brca1 Gene in Human Breast Carcinoma Mcf-7 Cells by Using Dna-Silver Nanoclusters, Methods and Applications in Fluorescence (2018)
Other Related Docs
9. Fast and Selective Whole Cell Detection of Staphylococcus Aureus Bacteria in Food Samples by Paper Based Colorimetric Nanobiosensor Using Peroxidase-Like Catalytic Activity of Dna-Au/Pt Bimetallic Nanoclusters, Microchemical Journal (2020)
10. Environmental Applications of Luminescent Metal Nanoclusters, Luminescent Metal Nanoclusters: Synthesis# Characterization# and Applications (2022)
11. Colorimetric Biosensor for Phenylalanine Detection Based on a Paper Using Gold Nanoparticles for Phenylketonuria Diagnosis, Microchemical Journal (2021)
12. Recent Advances in Optical Biosensors for Specific Detection of E. Coli Bacteria in Food and Water, Food Control (2022)
13. Colorimetric and Fluorescent Dual-Biosensor Based on Zirconium and Preasodium Metal-Organic Framework (Zr/Pr Mof) for Mirna-191 Detection, Heliyon (2024)
14. Paper Based Colorimetric Detection of Mirna-21 Using Ag/Pt Nanoclusters, Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy (2020)
15. Recent Developments in Dna Nanostructure-Based Biosensors for the Detection of Melamine Adulteration in Milk, Microchemical Journal (2023)
16. Smart Fluorescence Aptasensor Using Nanofiber Functionalized With Carbon Quantum Dot for Specific Detection of Pathogenic Bacteria in the Wound, Talanta (2022)
17. A Colorimetric Paper Sensor for Citrate As Biomarker for Early Stage Detection of Prostate Cancer Based on Peroxidase-Like Activity of Cysteine-Capped Gold Nanoclusters, Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy (2019)
18. A Novel Brca1 Gene Deletion Detection in Human Breast Carcinoma Mcf-7 Cells Through Fret Between Quantum Dots and Silver Nanoclusters, Journal of Pharmaceutical and Biomedical Analysis (2018)
19. Colorimetric and Energy Transfer Based Fluorometric Turn-On Method for Determination of Microrna Using Silver Nanoclusters and Gold Nanoparticles, Microchimica Acta (2018)
20. Sensitive Colorimetric Aptasensor Based on Peroxidase-Like Activity of Zrpr-Mof to Detect Salmonella Typhimurium in Water and Milk, Food Control (2023)
21. A Novel Dual-Mode and Label-Free Aptasensor Based Methodology for Breast Cancer Tissue Marker Targeting, Sensors and Actuators# B: Chemical (2020)
22. Microrna Sensing Using Integrating Dna-Functionalized Hydrogels With Aggregation-Induced Emission of Silver Nanoclusters, Sensors and Actuators B: Chemical (2023)
23. Sensitive Colorimetric Aptasensor Based on G-C3n4@Cu2o Composites for Detection of Salmonella Typhimurium in Food and Water, Microchimica Acta (2021)
24. Gold Nanorod Etching for Sensitive Aptamer-Mediated Colorimetric Detection of Escherichia Coli in Water, Microchemical Journal (2025)
25. Turn –On Fret-Based Cysteine Sensor by Sulfur-Doped Carbon Dots and Au Nanoparticles Decorated Ws2 Nanosheet, Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy (2022)
26. Fluorescence Self-Assembled Dna Hydrogel for the Determination of Prostate Specific Antigen by Aggregation Induced Emission, Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy (2023)
27. A New Colorimetric Assay for Sensitive Detection of Glucose-6-Phosphate Dehydrogenase Deficiency Based on Silver Nanoparticles, Nanotechnology (2022)
28. Label-Free Fluorescent Detection of Microrna-155 Based on Synthesis of Hairpin Dna-Templated Copper Nanoclusters by Etching (Top-Down Approach), Sensors and Actuators# B: Chemical (2017)
29. Fluorescence Turn-On Detection of Mirna-155 Based on Hybrid Ce-Mof/ Ptnps /Graphene Oxide Serving As Fluorescence Quencher, Journal of Photochemistry and Photobiology A: Chemistry (2022)
30. Oxidase-Like Catalytic Activity of Cys-Auncs Upon Visible Light Irradiation and Its Application for Visual Mirna Detection, Sensors and Actuators# B: Chemical (2018)
31. Fluorescent Turn on Sensing of Caffeine in Food Sample Based on Sulfur-Doped Carbon Quantum Dots and Optimization of Process Parameters Through Response Surface Methodology, Sensors and Actuators# B: Chemical (2018)
32. A Fluorometric Study on the Effect of Dna Methylation on Dna Interaction With Graphene Quantum Dots, Methods and Applications in Fluorescence (2019)
33. A Simple Smartphone-Assisted Paper-Based Colorimetric Biosensor for the Detection of Urea Adulteration in Milk Based on an Environment-Friendly Ph-Sensitive Nanocomposite, Analytica Chimica Acta (2023)
34. Virus-Directed Synthesis of Emitting Copper Nanoclusters As an Approach to Simple Tracer Preparation for the Detection of Citrus Tristeza Virus Through the Fluorescence Anisotropy Immunoassay, Sensors and Actuators# B: Chemical (2020)