Altered Expression of Leukemia Inhibitory Factor (Lif), Lifr, Gp130, and Il11 in the Embryo Implantation Site of Rat Model With Prenatal Androgen-Induced Polycystic Ovary Syndrome

Altered Expression of Leukemia Inhibitory Factor (Lif), Lifr, Gp130, and Il11 in the Embryo Implantation Site of Rat Model With Prenatal Androgen-Induced Polycystic Ovary Syndrome Publisher Pubmed

Javidan M¹ ; Changaei M¹ ; Ramezani Tehrani F² ; Mosaffa N³ ; Noroozzadeh M² ; Hosseinzadeh R¹ ; Rajaei S¹

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1. Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Reproductive Endocrinology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3. Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Biochemical and Biophysical Research Communications Published:2022

Abstract

Polycystic ovary syndrome (PCOS) is a complex endocrine disorder that represents infertility in many reproductive-age women. Reduced implantation of blastocyst was proposed as an etiology for infertility in this syndrome. In this regard, many candidate genes such as leukemia inhibitory factor (LIF), LIF receptor (LIFR), glycoprotein 130 (gp130), and interleukin 11 (IL11) were proposed to be disrupted. Investigation of these genes is not ethically approved in pregnant women with PCOS. In this study, we aimed to compare the expression of LIF, LIFR, gp130, and IL11 before and during different gestational days in uterine tissues of prenatally-androgenized rat models of PCOS with control rats. The rat model of polycystic ovary syndrome was created by the injection of testosterone during prenatal life. RNA extraction and cDNA synthesis from uterine tissues were performed in both prenatal induced PCOS and control rats. Expression of LIF, LIFR, gp130, and IL11 genes was compared before pregnancy (GD0) and during pregnancy on GD0.5, GD4.5, GD5.5, and GD8.5 between two study groups (n = 6 each group) using SYBR Green real-time PCR. The expression of the LIF mRNAs significantly decreased on GD4.5, 5.5, and 8.5 in the PCOS rats compared to the controls (P-values: 0.0483, 0.0152, and 0.0043). Additionally, decreased expression of LIFR and gp130 was observed on GD0.5 to 8.5 in PCOS rats compared to controls (P-values: 0.022, 0.0480, 0.0043, 0.0022 for LIFR and 0.0189, 0.0022, 0.0087, 0.0022 for gp130). Moreover, IL-11 mRNA levels decreased in the PCOS group compared to their controls both before (P-value:0.0362) and during the gestational period (P-values:0.0085, 0.0043, 0.0389, 0.0087). Reduced expression of LIF, LIFR, gp130, and IL11 in the rats with PCOS indicates a possible disruption in the implantation and decidualization stages in this syndrome. © 2022 Elsevier Inc.

2. Expression of Cyp19, Socs3, and Hoxa Genes in the Reproductive System of a Rat Model of Polycystic Ovary Syndrome, Iranian Journal of Endocrinology and Metabolism (2023)

3. Cardiac Function and Tolerance to Ischemia/Reperfusion Injury in a Rat Model of Polycystic Ovary Syndrome During the Postmenopausal Period, Life Sciences (2020)

4. Production and Characterization of Recombinant Human Leukemia Inhibitory Factor and Evaluation of Anti-Fertility Effects of Rabbit Anti-Rhlif in Balb/C Mice, Protein Expression and Purification (2020)

5. A Review on Cyp11a1, Cyp17a1, and Cyp19a1 Polymorphism Studies: Candidate Susceptibility Genes for Polycystic Ovary Syndrome (Pcos) and Infertility, Genes (2022)

6. Comparison of in Vitro Fertilization/Intracytoplasmic Sperm Injection Cycle Outcome in Patients With and Without Polycystic Ovary Syndrome: A Modified Poisson Regression Model, International Journal of Fertility and Sterility (2018)

7. Leukemia Inhibitory Factor Enhanced the Developmental and Implantation Compatibility of Mouse Embryos in Co-Culture With Human Endometrial Epithelial Cells, Reproductive and Developmental Medicine (2021)

8. Role of Gut Microbiome in Polycystic Ovary Syndrome, Journal of Mazandaran University of Medical Sciences (2021)

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Style	Citing Format
MLA	Javidan M, et al.. "Altered Expression of Leukemia Inhibitory Factor (Lif), Lifr, Gp130, and Il11 in the Embryo Implantation Site of Rat Model With Prenatal Androgen-Induced Polycystic Ovary Syndrome." Biochemical and Biophysical Research Communications, vol. 605, no. , 2022, pp. 24-30.
APA	Javidan M, Changaei M, Ramezani Tehrani F, Mosaffa N, Noroozzadeh M, Hosseinzadeh R, Rajaei S (2022). Altered Expression of Leukemia Inhibitory Factor (Lif), Lifr, Gp130, and Il11 in the Embryo Implantation Site of Rat Model With Prenatal Androgen-Induced Polycystic Ovary Syndrome. Biochemical and Biophysical Research Communications, 605(), 24-30.
Chicago	Javidan M, Changaei M, Ramezani Tehrani F, Mosaffa N, Noroozzadeh M, Hosseinzadeh R, Rajaei S. "Altered Expression of Leukemia Inhibitory Factor (Lif), Lifr, Gp130, and Il11 in the Embryo Implantation Site of Rat Model With Prenatal Androgen-Induced Polycystic Ovary Syndrome." Biochemical and Biophysical Research Communications 605, no. (2022): 24-30.
Harvard	Javidan M et al. (2022) 'Altered Expression of Leukemia Inhibitory Factor (Lif), Lifr, Gp130, and Il11 in the Embryo Implantation Site of Rat Model With Prenatal Androgen-Induced Polycystic Ovary Syndrome', Biochemical and Biophysical Research Communications, 605(), pp. 24-30.
Vancouver	Javidan M, Changaei M, Ramezani Tehrani F, Mosaffa N, Noroozzadeh M, Hosseinzadeh R, et al.. Altered Expression of Leukemia Inhibitory Factor (Lif), Lifr, Gp130, and Il11 in the Embryo Implantation Site of Rat Model With Prenatal Androgen-Induced Polycystic Ovary Syndrome. Biochemical and Biophysical Research Communications. 2022;605():24-30.
BibTex	@article{ author = {Javidan M and Changaei M and Ramezani Tehrani F and Mosaffa N and Noroozzadeh M and Hosseinzadeh R and Rajaei S}, title = {Altered Expression of Leukemia Inhibitory Factor (Lif), Lifr, Gp130, and Il11 in the Embryo Implantation Site of Rat Model With Prenatal Androgen-Induced Polycystic Ovary Syndrome}, journal = {Biochemical and Biophysical Research Communications}, volume = {605}, number = {}, pages = {24-30}, year = {2022} }
RIS	TY - JOUR AU - Javidan M AU - Changaei M AU - Ramezani Tehrani F AU - Mosaffa N AU - Noroozzadeh M AU - Hosseinzadeh R AU - Rajaei S TI - Altered Expression of Leukemia Inhibitory Factor (Lif), Lifr, Gp130, and Il11 in the Embryo Implantation Site of Rat Model With Prenatal Androgen-Induced Polycystic Ovary Syndrome JO - Biochemical and Biophysical Research Communications VL - 605 IS - SP - 24 EP - 30 PY - 2022 ER -

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