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Differences in Gene Expression of Enzymes Involved in Branched-Chain Amino Acid Metabolism of Abdominal Subcutaneous Adipose Tissue Between Pregnant Women With and Without Pcos Publisher Pubmed



Hajitarkhani S1, 2 ; Moini A3, 4, 5 ; Hafezi M3 ; Shahhoseini M6, 7, 8 ; Alizadeh A1
Authors
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Authors Affiliations
  1. 1. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
  2. 2. Department of Cellular and Molecular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University Science and Culture, Tehran, Iran
  3. 3. Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
  4. 4. Breast Disease Research Center (BDRC), Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Gynecology and Obstetrics, Arash Women's Hospital, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
  7. 7. Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
  8. 8. Department of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran

Source: Taiwanese Journal of Obstetrics and Gynecology Published:2021


Abstract

Objective: Polycystic ovary syndrome (PCOS) appears to be a common endocrine disorder of women in reproductive age. Adipose tissue (AT) is known as an active tissue in the metabolism of branched-chain amino acids (BCAA; Valine, Leucine, and Isoleucine) that they have associated with blood BCAA levels is a prognostic factor for insulin-resistant. Although the crucial roles of AT in women suffering from PCOS was reported, little information exists on the BCAA metabolism in AT of PCOS women. The aim was to assess and compare the expression of BCAAs metabolism pathway genes in abdominal subcutaneous AT of pregnant women with PCOS and non-PCOS pregnant women. Materials and methods: AT samples from 13 PCOS were compared with samples collected from 6 non-PCOS women, all of whom underwent caesarean. Quantitative real-time PCR technique was used for gene expression of branched chain aminotransferase 2 mitochondrial (BCAT2), branched chain ketoacid dehydrogenase E1-alpha (BCKDHA), branched chain ketoacid dehydrogenase E1-Beta (BCKDHB), dihydrolipoamide branched chain transacylase E2 (DBT), dihydrolipoamide dehydrogenase E3 (DLD), branched chain ketoacid dehydrogenase kinase (BCKDK), Data were analyzed using t-test or U-test. Results: No significant differences were found in age and body mass index (BMI) between non-PCOS and PCOS women. The mRNA level of BCAT2 and DLD in PCOS group was not significantly different from non-PCOS group whereas mRNA level of BCKDHB and DBT was significantly increased in PCOS group (P < 0.0001). In contrast, mRNA level of BCKDHA (P = 0.0001) and BCKDK (P < 0.0001) was significantly decreased in PCOS group. Conclusion: The alterations in gene expressions involved BCAA metabolism in age-matched and BMI- matched non-PCOS and PCOS pregnant women at delivery day was shown which warrants further studies regards functional activity. More attention should be given to AT of PCOS mothers that was previously ignored. © 2021
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1. Differences in Fatty Acid Profiles and Desaturation Indices of Abdominal Subcutaneous Adipose Tissue Between Pregnant Women With and Without Pcos, Adipocyte (2020)
2. Differences in Expression of Genes Related to Steroidgenesis in Abdominal Subcutaneous Adipose Tissue of Pregnant Women With and Without Pcos; a Case Control Study, BMC Pregnancy and Childbirth (2021)
Experts (# of related papers)
Ashraf Moini (2)