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Evaluation of Enkephalin-Degrading Enzymes in Sperm From Heroin-Addicted Men Publisher



Rezaeimojaz S1 ; Nazmara Z1 ; Najafi M2, 3 ; Movahedin M4 ; Zandieh Z2, 5 ; Shirinbayan P6 ; Roshanpajouh M7 ; Asgari HR1 ; Abbasi M8 ; Koruji M1, 2
Authors
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Authors Affiliations
  1. 1. Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  4. 4. Anatomical Sciences Department, School of Medicine, Tarbiat Modares University, Tehran, Iran
  5. 5. Shahid Akbarabadi Clinical Research Development Unit (ShACRDU), Iran University of, Medical Sciences, Tehran, Iran
  6. 6. Pediatric Neuro-Rehabilitation Research Center, The University of Social Welfare and Rehabilitation Sciences, Tehran, Iran
  7. 7. School of Behavioral Sciences and Mental Health, Tehran Psychiatry Institute, Iran University of Medical Sciences, Tehran, Iran
  8. 8. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: International Journal of Fertility and Sterility Published:2020


Abstract

Background: The aim of this study was to investigate two enkephalin-degrading enzymes, aminopeptidase N (APN/ CD13) and endopeptidase (NEP/CD10), gene and protein expression levels in sperm samples of fertile and heroin-addicted men, and the correlation between their expressions and semen quality. Materials and Methods: In this case-controlled study, semen was collected from 24 normozoospermic healthy (as a control group) and 24 heroin-addicted men donors (as case or addiction group). Sperm cells isolated by Cook Medical gradient (40-80%) and followed up by swim-up techniques were used for real-time quantitative polymerase chain reaction (qPCR) and flow cytometry techniques to assess APN/CD13 and NEP/CD10 genes and proteins subsequently. Semen parameters were analyzed by computer-assisted sperm analysis. Results: The findings revealed that there were significant differences in sperm total motility (41.07 ± 3.63 vs. 63.03 ± 3.31 %, P=0.0001), progressive motility (35.21 ± 2.64 vs. 20.93 ± 3.22%, P=0.001) and viability (69.9 ± 4.69 vs. 86.81 ± 1.26 %, P=0.002) in the addicted group vs. control ones. APN and NEP gene expression levels in the addicted group decreased compared with the control ones (1.00 ± 0.67 vs. 0.36 ± 0.13, P= 0.008 and 1.07 ± 0.11 vs. 0.52 ± 0.12 0.002, respectively). Flow cytometry analysis showed that the average percent of APN/CD13 in heroin consumers significantly decreased compared with the healthy ones, while NEP/CD10 rate between two groups was similar. We also observed that duration of drug dependence is correlated with sperm viability (r=-0.627, P=0.016) and motility (r=-0.410, P=0.05), NEP (r=-0.434, P= 0.049), and APN (r=-0.641, P=0.002) gene expression levels. Conclusion: We conclude that semen quality and enkephalin-degrading enzymes were altered in heroin-addicted men. other confirming the internal validity of our estimates. © Royan Institute (ACECR). All rights reserved.
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