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Extraction and Identification of Two Flavonoids in Phlomoides Hyoscyamoides As an Endemic Plant of Iran: The Role of Quercetin in the Activation of the Glutathione Peroxidase, the Improvement of the Hydroxyproline and Protein Oxidation in Bile Duct-Ligated Rats Publisher Pubmed



Doustimotlagh AH1, 2 ; Taheri S3 ; Mansourian M1, 4 ; Eftekhari M5
Authors
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Authors Affiliations
  1. 1. Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
  2. 2. Department of Clinical Biochemis-try, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
  3. 3. Chemistry & Chemical Engineering Research Center of Iran, P.O. Box 14335-186, Tehran, Iran
  4. 4. Department of Pharmacology, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
  5. 5. Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sci-ences, Tehran, Iran

Source: Current Computer-Aided Drug Design Published:2020


Abstract

Background: Cholestatic liver disease, a serious chronic condition that develops progressive hepatic degeneration through free radicals. Objective: The present study was designed to extract and identify two flavonoids in Phlomoides hyoscya-moides plant, native to Iran and evaluate the role of quercetin identified on the liver injury among bile duct-ligated rats. Methods: This study was conducted on 25 male Wistar rats within three groups of sham control, mere bile duct-ligated, and bile duct-ligated with quercetin. The bile duct-ligated animals received quercetin at a dose of 50 mg/kg/day for 10 days, followed by biochemical tests, oxidative stress markers, activity of antioxi-dant enzymes and hematoxylin and eosin staining. Molecular docking was used to explore the interactive behavior of quercetin with glutathione peroxidase. Results: According to analyses of the obtained extract, two main active ingredients of P. hyoscyamoides were rutin and quercetin. Bile duct-ligated group showed a significant liver necrosis, a clear increase in plasma and tissue oxidative stress parameters, and a decrease in glutathione peroxidase activity as com-pared to sham control group. Quercetin injection in bile duct-ligated rats resulted in significant decrease in hydroxyproline, protein carbonyl and histopathologic indexes and significant increase in glutathione perox-idase activity (P-value≤0.05). Based on the molecular docking, the quercetin was able to regulate the gluta-thione peroxidase activity. Conclusion: The quercetin acts as an enzyme inducer by renewing the glutathione peroxidase activity and inhibiting the oxidation of proteins and hence decreases the oxidative stress. These results could be a sign of confirming the positive role of quercetin in attenuating the liver damage and degeneration. © 2020 Bentham Science Publishers.
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