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Fabrication and Characterization of Electrospun Laminin-Functionalized Silk Fibroin/Poly(Ethylene Oxide) Nanofibrous Scaffolds for Peripheral Nerve Regeneration Publisher Pubmed



Rajabi M1 ; Firouzi M2 ; Hassannejad Z3, 4 ; Haririan I5 ; Zahedi P1
Authors
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Authors Affiliations
  1. 1. Department of Polymer, School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran
  2. 2. Tissue Repair Laboratory, Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran
  3. 3. Pediatric Urology and Regenerative Medicine Research Center, Children's Hospital Medical Center, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Pharmaceutical Biomaterials and Medical Biomaterials Research Center, Faculty of Pharmacy and Department of Pharmaceutics, Tehran University of Medical Sciences, Tehran, P. O. Box: 14155-6451, Tehran, Iran

Source: Journal of Biomedical Materials Research - Part B Applied Biomaterials Published:2018


Abstract

The peripheral nerve regeneration is still one of the major clinical problems, which has received a great deal of attention. In this study, the electrospun silk fibroin (SF)/poly(ethylene oxide) (PEO) nanofibrous scaffolds were fabricated and functionalized their surfaces with laminin (LN) without chemical linkers for potential use in the peripheral nerve tissue engineering. The morphology, surface chemistry, thermal behavior and wettability of the scaffolds were examined to evaluate their performance by means of scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC) and water contact angle (WCA) measurements, respectively. The proliferation and viability of Schwann cells onto the surfaces of SF/PEO nanofibrous scaffolds were investigated using SEM and thiazolyl blue (MTT) assay. The results showed an improvement of SF conformation and surface hydrophilicity of SF/PEO nanofibers after methanol and O 2 plasma treatments. The immunostaining observation indicated a continuous coating of LN on the scaffolds. Improving the surface hydrophilicity and LN functionalization significantly increased the cell proliferation and this was more prominent after 5 days of culture time. In conclusion, the obtained results revealed that the electrospun LN-functionalized SF/PEO nanofibrous scaffold could be a promising candidate for peripheral nerve tissue regeneration. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1595–1604, 2018. © 2017 Wiley Periodicals, Inc.
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