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Toxicity Effects of Agzno Nanoparticles and Rifampicin on Mycobacterium Tuberculosis Into the Macrophage Publisher Pubmed



Jafari A1 ; Jafari Nodooshan S2 ; Safarkar R3 ; Movahedzadeh F4, 5 ; Mosavari N6 ; Novin Kashani A7 ; Dehghanpour M6 ; Kamalzadeh M8 ; Rasouli Koohi S1 ; Fathizadeh S1 ; Majidpour A1, 9
Authors
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Authors Affiliations
  1. 1. Institute of Immunology and Infectious Diseases (IIID), Anti-Microbial Resistance Research Center, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Microbiology, Islamic Azad University, Ardabil Branch, Ardabil, Iran
  4. 4. University of Illinois at Chicago, Institute for Tuberculosis Research, Chicago, IL, United States
  5. 5. Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy University of Illinois at Chicago, Chicago, IL, United States
  6. 6. Reference Laboratory for Bovine Tuberculosis, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization, Tehran, Iran
  7. 7. College of Nursing, University of Illinois at Springfield, Chicago, IL, United States
  8. 8. Quality Control, Department, Razi Vaccine and Research Institute, Agricultural Research Education and Extension Organization, Karaj, Iran
  9. 9. Department of, Infectious, Diseases, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

Source: Journal of Basic Microbiology Published:2018


Abstract

The World Health Organization acknowledges tuberculosis as a global threat. Tuberculosis infection is one of the top 10 causes of death worldwide. Nanotechnology and microbiology researchers are looking for new and safe nano drugs for eliminating Mycobacterium tuberculosis, the causative agent of tuberculosis. In this study, AgZnO nano-crystals (AgZnONCs) is synthesized via the decomposition of the precursor of oxalate method. Characterization of AgZnONCs were evaluated. Next, various concentrations of AgZnONCs, as well AgZnONCs+Rifampicin, were prepared. The MTT assay was employed to study the viability of human macrophage cell lines (THP-1) exposed to AgZnONCs. The bactericidal effects of AgZnONCs and AgZnONCs+Rifampicin were studied by Minimum Bactericidal Concentration (MBC) test. Subsequently, THP-1 were infected by H37Rv strain of M. tuberculosis (H37RvMtb). Also, bactericidal effects of AgZnONCs and AgZnONCs+Rifampicin were compared with ex-vivo conditions. The MBC of AgZnONCs and AgZnONCs+Rifampicin were ratios of 1:4 and 1:32 respectively (p-value <0.05). Also, more than 50% and 80% of THP-1 were alive in ratios of 1:4 and 1:32 in the presence of AgZnONCs, respectively. All phagocytic H37RvMtb were killed in the presence of AgZnONCs+Rifampicin (p-value <0.05), while AgZnONCs were not able to kill all the H37RvMtb (p-value >0.05). This study showed that, AgZnONCs+Rifampicin has the most anti-tubercular behavior with respect to the macrophages. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
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