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Green and Simple Synthesis of Silver Nanoparticles by Aqueous Extract of Perovskia Abrotanoides: Characterization, Optimization and Antimicrobial Activity Publisher Pubmed



Mirsadeghi S1 ; Koudehi MF2 ; Rajabi HR3 ; Pourmortazavi SM2
Authors
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Authors Affiliations
  1. 1. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, 1411713137, Iran
  2. 2. Faculty of Chemistry and Chemical Engineering, Malek Ashtar University of Technology, Tehran, Iran
  3. 3. Chemistry Department, Yasouj University, Yasouj, 75918-74831, Iran

Source: Current Pharmaceutical Biotechnology Published:2020


Abstract

Background: Herein, we report the biosynthesis procedure to prepare silver nanoparticles as reduction and capping agents with the aqueous plant extract of Perovskia abrotanoides. Methods: The therapeutic application of silver nanoparticles entirely depends on the size and shape of the nanoparticles therefore, their control during the synthesis procedure is so important. The effects of synthesis factors, for example, silver ion concentration, the mass of plant extract, reaction time and extraction temperature, on the size of silver particles were considered and optimized. Several analytical methods were used for the characterization of silver NPs including FT-IR and UV–Vis spectrophotom-eter, XRD and SEM. Results: The results showed that the mean size of the silver particles was about 51 nm. Moreover, the antibacterial properties of biosynthesized silver NPs were investigated by the minimum inhibitory con-centration, minimum bactericidal concentration, and Well-diffusion tests. The minimum inhibitory con-centration/minimum bactericidal concentration values of silver NPs and aqueous plant extract versus Gram-positive bacteria (Staphylococcus aureus and Bacillus cereus) and Gram-negative bacteria (E. coli) were 3.03/0.00, 1.20/0.01, 3.06/0.00, 0.98/1.04, 1.00/0.05 and 1.30/0.03 (mg/mL), respectively. Conclusion: The antimicrobial activity study displayed that the synthesized silver nanoparticles by plant extract have better antimicrobial properties compared to aqueous plant extract of Perovskia abro-tanoides. © 2020 Bentham Science Publishers.
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