The Effects of Ethanol and Aqueous Extracts of Moringa Oleifera Leaves on Learning, Spatial Memory and Anxiety in Male Mice Publisher



Ghafelebashi M^{1, 2} ; Chahardehi AM¹ ; Hasannezhad B¹ ; Mohammadi H³ ; Alamuti MG¹ ; Tootoonchi S⁴ ; Hosseini Y¹ Show Affiliations
Source: Activitas Nervosa Superior Rediviva Published:2024

Abstract

OBJECTIVE: Moringa oleifera leaves are widely used in the treatment of a variety of disorders such as cognitive deficits. Several studies show that M. oleifera 's leaves, roots, and seeds all contain high levels of micronutrients, which enhance brain function. The objective of this study is to investigate and compare the effects of ethanolic and aqueous extracts of M. oleifera leaves on spatial memory and anxiety-like behavior at various doses. METHOD: Thirty male mice were randomly divided into five groups (n = 6) and administrated those extracts via intraperitoneal injection for 30 consecutive days. Behavioral assays were performed using the Barnes maze and elevated plus maze tests. RESULTS: No significant change was observed in the final body weight. Both aqueous and ethanol extracts lead to improved learning and memory by reducing errors. However ethanol extract at 200 mg/kg had a greater effect on improving learning and memory, and did not enhance anxiety compared to the other doses. CONCLUSION: Ethanol extract of M. oleifera leaves in high dose has a more positive cognitive effect rather than aqueous extract of M. oleifera leaves. Copyright © 2024 Activitas Nervosa Superior Rediviva.