A Highly Selective Semiconducting Polymer Dots-Based “Off–On” Fluorescent Nanoprobe for Iron, Copper and Histidine Detection and Imaging in Living Cells

A Highly Selective Semiconducting Polymer Dots-Based “Off–On” Fluorescent Nanoprobe for Iron, Copper and Histidine Detection and Imaging in Living Cells Publisher Pubmed

Chabok A¹ ; Shamsipur M² ; Yeganehfaal A³ ; Molaabasi F⁴ ; Molaei K¹ ; Sarparast M⁵

Show Affiliations

1. Department of Chemistry, Tarbiat Modares University, Tehran, Iran
2. Department of Chemistry, Razi University, Kermanshah, Iran
3. Department of Chemistry, Payame Noor University, Tehran, Iran
4. Department of Biomaterials and Tissue Engineering, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran
5. Department of Chemistry, Michigan State University, East Lansing, 48824-1322, MI, United States

Source: Talanta Published:2019

Abstract

Semiconducting polymer dots (PDs) hold a great promise as fluorescence nanoprobes, due to their photostability, biocompatibility, and high quantum yield. Herein, the synthesis and characterization of highly fluorescent PDs for selective and sensitive detection of Fe3+,Cu2+, and histidine (His) have been reported. First, carboxyl functionalized poly[(9,9-dioctylfluorenyl-2,7-diyl)-alt-co-(1,4-benzo-(2,1′,3)-thiadiazole)] (PFBT) PDs were synthesized through a nano-precipitation technique, and then they were functionalized by -COOH groups using 9-anthracenecarboxylic acid. The formation of PDs was proved using transmission electron microscopy, dynamic light scattering, and Fourier transform infrared (FTIR) spectroscopy analyses. The PDs exhibited a yellow fluorescence with a peak centered at 540 nm (photo-excited at 460 nm) with a quantum yield of 25%. The fluorescence of PDs significantly quenched in the presence of Cu2+ ion, and then selectively recovered upon addition of His, providing the possibility of constructing a sensitive Cu2+-His off-on fluorescent nanoprobe. The PDs exhibited a linear dynamic range for Cu2+ from 0.1 to 630 µmol L−1 with a limit of detection of 61.7 nmol L−1, and for Fe3+ from 0.1 to 720 µmol L−1 with a limit of detection of 58.1 nmol L−1. In addition, the PDs/Cu2+ probe showed a linear dynamic range for His from 0.1 to 920 µmol L−1 with a limit of detection of 79.6 nmol L−1. Besides, the prepared PDs/Cu2+ probe exhibited a promising potential for selective and sensitive sensing of His in blood serum and for intracellular imaging. © 2018 Elsevier B.V.

2. Fluorescent Nanoclusters for Imaging of Cells/Stem Cells, Methods in Molecular Biology (2020)

3. Shape-Controlled Synthesis of Luminescent Hemoglobin Capped Hollow Porous Platinum Nanoclusters and Their Application to Catalytic Oxygen Reduction and Cancer Imaging, Scientific Reports (2018)

4. Challenges and Opportunities of Using Fluorescent Metal Nanocluster-Based Colorimetric Assays in Medicine, ACS Omega (2023)

Style	Citing Format
MLA	Chabok A, et al.. "A Highly Selective Semiconducting Polymer Dots-Based “Off–On” Fluorescent Nanoprobe for Iron, Copper and Histidine Detection and Imaging in Living Cells." Talanta, vol. 194, no. , 2019, pp. 752-762.
APA	Chabok A, Shamsipur M, Yeganehfaal A, Molaabasi F, Molaei K, Sarparast M (2019). A Highly Selective Semiconducting Polymer Dots-Based “Off–On” Fluorescent Nanoprobe for Iron, Copper and Histidine Detection and Imaging in Living Cells. Talanta, 194(), 752-762.
Chicago	Chabok A, Shamsipur M, Yeganehfaal A, Molaabasi F, Molaei K, Sarparast M. "A Highly Selective Semiconducting Polymer Dots-Based “Off–On” Fluorescent Nanoprobe for Iron, Copper and Histidine Detection and Imaging in Living Cells." Talanta 194, no. (2019): 752-762.
Harvard	Chabok A et al. (2019) 'A Highly Selective Semiconducting Polymer Dots-Based “Off–On” Fluorescent Nanoprobe for Iron, Copper and Histidine Detection and Imaging in Living Cells', Talanta, 194(), pp. 752-762.
Vancouver	Chabok A, Shamsipur M, Yeganehfaal A, Molaabasi F, Molaei K, Sarparast M. A Highly Selective Semiconducting Polymer Dots-Based “Off–On” Fluorescent Nanoprobe for Iron, Copper and Histidine Detection and Imaging in Living Cells. Talanta. 2019;194():752-762.
BibTex	@article{ author = {Chabok A and Shamsipur M and Yeganehfaal A and Molaabasi F and Molaei K and Sarparast M}, title = {A Highly Selective Semiconducting Polymer Dots-Based “Off–On” Fluorescent Nanoprobe for Iron, Copper and Histidine Detection and Imaging in Living Cells}, journal = {Talanta}, volume = {194}, number = {}, pages = {752-762}, year = {2019} }
RIS	TY - JOUR AU - Chabok A AU - Shamsipur M AU - Yeganehfaal A AU - Molaabasi F AU - Molaei K AU - Sarparast M TI - A Highly Selective Semiconducting Polymer Dots-Based “Off–On” Fluorescent Nanoprobe for Iron, Copper and Histidine Detection and Imaging in Living Cells JO - Talanta VL - 194 IS - SP - 752 EP - 762 PY - 2019 ER -

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