Preparation and Characterization of a Monoclonal Antibody Against Morphine

Preparation and Characterization of a Monoclonal Antibody Against Morphine Publisher Pubmed

Kashanian S¹ ; Shams A¹ ; Ghahremani H² ; Paknejad M²

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1. Department of Immunology, School of Medicine, International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2. Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, 1417613151, Iran

Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Published:2015

Abstract

A monoclonal antibody (MAb) was produced by immunization of a BALB/c mouse with a conjugated morphine C6-hemisuccinated derivative (MHS) to cationized bovine serum albumin (cBSA). The hybridoma clones were screened by indirect ELISA using MHS-BSA. The best hybridoma clone was subcloned thrice by limiting dilution. This hybridoma was found to be of IgG2b class and subclass and contained lambda light chain. The affinity of the MAb to morphine was obtained 2.8×109 M-1. The titer of the cell culture supernatant was at least 1:800. The MAb was cross-reacted with codeine (100%) and apomorphine (16.5%), but not with heroin, naloxone, naltrexone, or papaverine. Morphine was conjugated to HRP using a mixed anhydride method and a direct competitive ELISA was designed using anti-morphine MAb. The assay was sensitive over the 50ng/mL to 5μg/mL concentration range. In conclusion, this MAb is useful for the development of immunoassays to measure morphine in urine. © Copyright 2015, Mary Ann Liebert, Inc. 2015.

2. Advances in Phage Display Technology for Drug Discovery, Expert Opinion on Drug Discovery (2015)

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Style	Citing Format
MLA	Kashanian S, et al.. "Preparation and Characterization of a Monoclonal Antibody Against Morphine." Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, vol. 34, no. 4, 2015, pp. 270-274.
APA	Kashanian S, Shams A, Ghahremani H, Paknejad M (2015). Preparation and Characterization of a Monoclonal Antibody Against Morphine. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 34(4), 270-274.
Chicago	Kashanian S, Shams A, Ghahremani H, Paknejad M. "Preparation and Characterization of a Monoclonal Antibody Against Morphine." Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 34, no. 4 (2015): 270-274.
Harvard	Kashanian S et al. (2015) 'Preparation and Characterization of a Monoclonal Antibody Against Morphine', Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 34(4), pp. 270-274.
Vancouver	Kashanian S, Shams A, Ghahremani H, Paknejad M. Preparation and Characterization of a Monoclonal Antibody Against Morphine. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 2015;34(4):270-274.
BibTex	@article{ author = {Kashanian S and Shams A and Ghahremani H and Paknejad M}, title = {Preparation and Characterization of a Monoclonal Antibody Against Morphine}, journal = {Monoclonal Antibodies in Immunodiagnosis and Immunotherapy}, volume = {34}, number = {4}, pages = {270-274}, year = {2015} }
RIS	TY - JOUR AU - Kashanian S AU - Shams A AU - Ghahremani H AU - Paknejad M TI - Preparation and Characterization of a Monoclonal Antibody Against Morphine JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy VL - 34 IS - 4 SP - 270 EP - 274 PY - 2015 ER -

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