The Anopheles Stephensi Odorant Binding Protein 1 (Asteobp1) Gene: A New Molecular Marker for Biological Forms Diagnosis Publisher Pubmed



Gholizadeh S^{1, 2} ; Firooziyan S² ; Ladonni H³ ; Hajipirloo HM⁴ ; Djadid ND⁵ ; Hosseini A⁶ ; Raz A⁵ Show Affiliations
Source: Acta Tropica Published:2015

Abstract

Anopheles (Cellia) stephensi Liston 1901 is known as an Asian malaria vector. Three biological forms, namely mysorensis, intermediate, and type have been earlier reported in this species. Nevertheless, the present morphological and molecular information is insufficient to diagnose these forms. During this investigation, An. stephensi biological forms were morphologically identified and sequenced for odorant-binding protein 1 (Obp1) gene. Also, intron I sequences were used to construct phylogenetic trees. Despite nucleotide sequence variation in exon of AsteObp1, nearly 100% identity was observed at the amino acid level among the three biological forms. In order to overcome difficulties in using egg morphology characters, intron I sequences of An. stephensi Obp1 opens new molecular way to the identification of the main Asian malaria vector biological forms. However, multidisciplinary studies are needed to establish the taxonomic status of An. stephensi. © 2015 .