Evaluation of Fosfomycin Activity Against Extended Spectrum Beta Lactamase (Esbl) Producing Enterobacteriaceae Isolated From Three Centers of Tehran, Iran Publisher Pubmed



Ghanavati R¹ ; Ohadi E¹ ; Kazemian H^{2, 3} ; Yazdani F⁴ ; Torki A¹ ; Kalani BS¹ ; Irajian G¹ Show Affiliations
Source: Recent Patents on Anti-Infective Drug Discovery Published:2018

Abstract

Background: Rising rates of antimicrobial resistance among Enterobacteriaceae limit the use of reliably active forms of available drugs. The aim of this study was to investigate the prevalence of fosfomycin (US6794490B2) resistance gene among ESBL producing isolates in Iran. Method: We tested 355 isolates of Enterobacteriacea collected from various clinical samples including urine, wounds, blood and other sources during June 2016 to July 2017. Antibiotic sensitivity and Extended Spectrum Beta Lactamase (ESBL) production were tested using agar dilution method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. ESBL genes (bla TEM , bla SHV ,bla CTX-M ), plasmid-encoded fosfomycin resistance genes (fosA, fosB, fosA3 and fosC2) and chromosomal mutations (murA, glpT, uhpT) were detected by Polymerase Chain Reaction (PCR). Results: In this study, 151 of the 355 isolates were ESBL-positive. bla CTX-M (77%) was the most common gene followed by bla SHV (70%) and bla TEM (58%), either alone or in combination. Eighty nine percent (132/151) of the ESBL-positive isolates were MDR. Antimicrobial susceptibility rates were higher for fosfomycin (92.8%) and imipenem (35.5%) among ESBL-positive isolates. None of the ESBL-positive isolates harbored any mutations or plasmid-mediated fosfomycin resistance determinants. Conclusion: In conclusion, fosfomycin showed good antimicrobial activity against. © 2018 Bentham Science Publishers.